A calcium sensor-regulated protein kinase, CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19, is required for pollen tube growth and polarity.
A calcium sensor-regulated protein kinase, CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19, is required for pollen tube growth and polarity.
复制标题
花粉管生长和极性需要钙传感器调节的蛋白激酶，钙调神经磷酸酶 B 样蛋白相互作用蛋白激酶 19。
DOI：
10.1104/pp.114.256065
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发表时间：
2015
期刊：
影响因子：
7.4
通讯作者：
Luan Sheng
中科院分区：
文献类型：
--
作者：
Zhou Liming;Lan Wenzhi;Chen Binqing;Fang Wei;Luan Sheng
钙在花粉管尖端的生长中起着至关重要的作用。然而，人们对所涉及的信号通路的分子基础知之甚少。在这里，我们通过对CIPK家族成员的功能调查，确定拟南芥（Arabidopsis thaliana）钙调磷酸酶B样蛋白相互作用蛋白激酶19（CIPK19）是花粉管生长的重要元素。 CIPK19基因在花粉粒和花粉管中特异性表达，其过度表达会导致花粉管生长严重丧失极性。体外和体内花粉管生长测定均证明，在 CIPK19 功能丧失突变体中，花粉管生长和极性显着受损。遗传分析表明 CIPK19 的破坏导致男性特异性的传播缺陷。此外，CIPK19 过表达引起的极性丧失与整个凸出尖端的胞质 Ca2+ 升高有关，而 Ca2+ 流入阻滞剂 LaCl3 可以挽救 CIPK19 过表达诱导的生长抑制。我们的结果表明 CIPK19 可能通过其调节 Ca2+ 内流的潜在功能参与维持 Ca2+ 稳态。
Calcium plays an essential role in pollen tube tip growth. However, little is known concerning the molecular basis of the signaling pathways involved. Here, we identified Arabidopsis (Arabidopsis thaliana) CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19 (CIPK19) as an important element to pollen tube growth through a functional survey for CIPK family members. TheCIPK19gene was specifically expressed in pollen grains and pollen tubes, and its overexpression induced severe loss of polarity in pollen tube growth. In the CIPK19 loss-of-function mutant, tube growth and polarity were significantly impaired, as demonstrated by both in vitro and in vivo pollen tube growth assays. Genetic analysis indicated that disruption ofCIPK19resulted in a male-specific transmission defect. Furthermore, loss of polarity induced by CIPK19 overexpression was associated with elevated cytosolic Ca2+throughout the bulging tip, whereas LaCl3, a Ca2+influx blocker, rescued CIPK19 overexpression-induced growth inhibition. Our results suggest that CIPK19 may be involved in maintaining Ca2+homeostasis through its potential function in the modulation of Ca2+influx.