Evidence for binding of dirhodium bis-acetate units to adjacent GG and AA sites on single-stranded DNA
Evidence for binding of dirhodium bis-acetate units to adjacent GG and AA sites on single-stranded DNA
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DOI：
10.1021/ja992800j
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发表时间：
2000-01-12
影响因子：
15
通讯作者：
Allison, J
中科院分区：
文献类型：
--
作者：
Asara, JM;Hess, JS;Allison, J
四乙酸二钠是一种抗肿瘤活性化合物，已知可抑制细胞DNA合成，但对其与核酸的相互作用知之甚少。结合基质辅助激光解吸/电离质谱（MALDI MS）和酶切实验，确定了双乙酸二钠单元在短DNA链上与AA和GG位点形成加合物。在添加精胺的辅助下，MALDI MS检测了dirhodium/DNA加合物，但无需特殊的样品处理即可获得相应顺铂修饰DNA链的高质量质谱。
Dirhodium tetraacetate is an antitumor active compound that is known to inhibit cellular DNA synthesis, but relatively little is known about its interactions with nucleic acids. A combination of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and enzymatic digestion experiments has established that the dirhodium bis-acetate unit forms an adduct with AA and GG sites in short DNA strands. The MALDI MS detection of the dirhodium/DNA adduct was aided by the addition of spermine, but no special sample treatment was required to obtain high quality mass spectra of the corresponding cisplatin-modified DNA strands.