Oxidized ApoC1 on MALDI-TOF and glycated-ApoA1 band on gradient gel as potential diagnostic tools for atherosclerotic vascular disease
Oxidized ApoC1 on MALDI-TOF and glycated-ApoA1 band on gradient gel as potential diagnostic tools for atherosclerotic vascular disease
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DOI：
10.1016/j.cca.2012.10.017
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发表时间：
2013-05-01
影响因子：
5
通讯作者：
Chen, Chao-Jung
中科院分区：
文献类型：
--
作者：
Chang, Chiz-Tzung;Liao, Hsin-Yi;Chen, Chao-Jung
背景资料：动脉粥样硬化性血管病（ASVD），包括冠状动脉疾病、缺血性卒中和外周血管疾病，是普通人群和高危患者最常见的死亡原因;糖尿病（DM）、尿毒症（UM）、原发性高脂血症（HP）患者发生ASVD的风险增加。为了鉴定用于早期预测ASVD的新生物标志物，来自疾病患者（ASVD）和风险增加但未记录ASVD的患者的HDL样本结果：MALDI-TOF法检测到ASVD患者血清中ApoC 1特异性氧化。在Bis-Tris梯度凝胶上，非ASVD ApoA 1显示为2条不同的条带A和B。ApoA 1的C带仅在ASVD患者中出现。ApoA 1的异常C带的特征在于高水平的糖化和氧化。结论：APoA 1蛋白C带的糖基化和氧化程度均高于非ASVD组，APoA 1蛋白C带的糖基化和氧化程度均高于非ASVD组，APoC 1蛋白C带的糖基化和氧化程度均高于非ASVD组，APoA 1蛋白C带的糖基化和氧化程度均高于非ASVD组。(c)出版社：Elsevier B. V.
Background: Atherosclerotic vascular disease (ASVD), including coronary artery disease, ischemic stroke, and peripheral vascular disease, is the most common cause of death both in the general population and in high-risk patients; patients with diabetes mellitus (DM), uremia (UM), primary hyperlipidemia (HP) have increased risks for developing ASVD.Methods: To identify new biomarkers for early prediction of ASVD, HDL samples from patients with disease (ASVD) and patients with increased risks but no documented ASVD (non-ASVD) were collected for Bis-Tris gradient gel and MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) analyses.Results: Oxidation of ApoC1 was detected specifically in ASVD samples by MALDI-TOF. On the Bis-Tris gradient gel, non-ASVD ApoA1 was displayed into 2 distinct bands A and B. An additional C band of ApoA1 appeared exclusively in ASVD patients. The extraordinary C band of ApoA1 was characterized by high levels of glycation and oxidation. MALDI-TOF analyses of ApoA1 peptides after trypsin digestion confirmed higher levels of glycation and oxidation levels in the ASVD than non-ASVD samples.Conclusions: Gel identification of the highly-glycated and oxidized C band of ApoA1 and MALDI-TOF detection of oxidized ApoC1 in HDL may provide a new approach for early ASVD diagnosis. (c) 2012 Published by Elsevier B.V.