PHAGOCYTIC PROCESSING OF BACTERIAL-ANTIGENS FOR CLASS-I MHC PRESENTATION TO T-CELLS
PHAGOCYTIC PROCESSING OF BACTERIAL-ANTIGENS FOR CLASS-I MHC PRESENTATION TO T-CELLS
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DOI：
10.1038/361359a0
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发表时间：
1993-01-28
期刊：
影响因子：
64.8
通讯作者：
HARDING, CV
中科院分区：
文献类型：
--
作者：
PFEIFER, JD;WICK, MJ;HARDING, CV
I类主要组织相容性复合体(MHC)分子呈递抗原，这些抗原在呈递细胞内产生，或从空泡系统穿透到胞浆中进行处理。大多数外源抗原处理的研究都使用了可溶性抗原，这些抗原不能有效地由I类MHC分子1呈递，也不能在体内引发CD8 T细胞反应。但是，尚不清楚胞浆穿透机制的颗粒抗原制剂也可以在活体2-7中诱导CD8 T细胞反应。我们在这里报道，细菌的吞噬作用没有胞浆穿透的机制，也导致I类MHC分子呈递细菌抗原。此外，这种机制对环己酰亚胺和灯盏花素A具有抵抗力，这两种物质阻止了经典的I类加工途径。这些结果提示了外源性吞噬细胞抗原的一种新的空泡I类处理途径。
CLASS I major histocompatibility complex (MHC) molecules present antigens that are produced within the presenting cell or penetrate from the vacuolar system into the cytosol for processing. Most studies of exogenous antigen processing have used soluble antigens, which are not efficiently presented by class I MHC molecules1 and do not elicit CD8 T-cell responses in vivo. But particulate antigen preparations with no known mechanism for cytosolic penetration can also elicit CD8 T-cell responses in vivo2-7. We report here that phagocytosis of bacteria with no mechanism for cytosolic penetration also results in presentation of bacterial antigens by class I MHC molecules. Moreover, this mechanism is resistant to cycloheximide and Brefeldin A, which block the classical class I processing pathway. These results suggest a novel vacuolar class I processing pathway for exogenous phagocytic antigens.