Associations between antimicrobial resistance genes in fecal generic Escherichia coli isolates from cow-calf herds in western Canada
Associations between antimicrobial resistance genes in fecal generic Escherichia coli isolates from cow-calf herds in western Canada
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DOI：
10.1128/aem.02505-07
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发表时间：
2008-06-01
影响因子：
4.4
通讯作者：
Boerlin, Patrick
中科院分区：
文献类型：
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作者：
Gow, Sheryl P.;Waldner, Cheryl L.;Boerlin, Patrick
本研究的目的是研究从加拿大西部77个奶牛群中获得的207个粪便类属大肠杆菌分离株中抗生素耐药性（AMR）的遗传决定因素之间的关联。23个耐药基因对应于6个不同的抗菌素家族进行了评估，使用DNA杂交和PCR。研究样本（207株分离株）中最常见的耐药基因为sul 2（48.3%）、泰特（B）（45.4%）和ant（3“）-Ia（aadA 1）（19.3%）。几个统计学显着的关联检测耐药基因。观察到的最强关联是氯霉素（catI）和甲氧苄啶（dhfrI）抗性基因之间的关联（比值比[OR] = 214; P = 0.0001），磺胺（sul 1）和氯霉素（catI）（OR = 96.9; P = 0.0001），链霉素[ant（3“）-Ia（aadA 1）]和甲氧苄啶（dhfr I）磺胺（sul 1）和链霉素[ant（3“）-Ia（aadA 1）]（OR = 79.3; P = 0.0001）;四环素[泰特（B）]和磺胺（sul 2）（OR = 25.7; P = 0.0001）。本研究中检测的每个非氨基糖苷类抗生素家族对应的至少一个耐药基因与两个氨基糖苷类耐药基因ant（X）-Ia（aadA 1）和aph（3 '）-Ia相关。本研究中描述的基因之间的多重强关联和关联的多样性表明了奶牛-小牛群中抗性基因选择的复杂性，在规划奶牛-小牛操作的AMR控制实践时应予以考虑。
The objective of this study was to examine associations among the genetic determinants of antimicrobial resistance (AMR) in 207 fecal generic Escherichia coli isolates obtained from 77 cow-calf herds in western Canada. Twenty-three resistance genes corresponding to six different antimicrobial families were assessed using DNA hybridization and PCR. The most common resistance genes in the study sample (207 isolates) were sul2 (48.3%), tet(B) (45.4%), and ant(3 '')-Ia (aadA1) (19.3%). Several statistically significant associations between the examined resistance genes were detected. The strongest associations observed were those between genes for resistance to chloramphenicol (catI) and trimethoprim (dhfrI) (odds ratio [OR] = 214; P = 0.0001), sulfonamide (sul1) and chloramphenicol (catI) (OR = 96.9; P = 0.0001), streptomycin [ant(3 '') -Ia (aadA1)] and trimethoprim (dhfrI) (OR = 96.2; P = 0.0001), sulfonamide (sul1) and streptomycin [ant(3 '')-Ia (aadA1)] (OR = 79.3; P = 0.0001), and tetracycline [tet(B)] and sulfonamides (sul2) (OR = 25.7; P = 0.0001). At least one of the resistance genes corresponding to each nonaminoglycoside family of antimicrobials examined in this study was associated with the two aminoglycoside resistance genes ant(X)-Ia (aadA1) and aph(3')-Ia. The multiple, strong associations between genes and the diverse nature of the associations described in this study demonstrate the complexity of resistance gene selection in cow-calf herds and should be considered in the planning of AMR control practices for cow-calf operations.