Cyclic force upregulates mechano-growth factor and elevates cell proliferation in 3D cultured skeletal myoblasts
Cyclic force upregulates mechano-growth factor and elevates cell proliferation in 3D cultured skeletal myoblasts
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循环力上调机械生长因子并提高 3D 培养骨骼肌成肌细胞的细胞增殖
DOI：
10.1016/j.abb.2009.08.016
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发表时间：
2009-10-15
影响因子：
3.9
通讯作者：
Yang, Pu
中科院分区：
文献类型：
--
作者：
Li, Yu;Zhao, Zhihe;Yang, Pu
机械生长因子（Mechano-growth factor， MGF）是胰岛素生长因子- 1 （IGF-I）的剪接变体，近年来受到越来越多的关注。在这项研究中，我们使用一个新建立的系统，三维培养大鼠骨骼肌母细胞，并加载不同大小或持续时间的循环单轴拉伸应变。检测各组细胞增殖指数（PI）及MGF、IGF-IEa、整合素β mRNA表达。结果表明：(1)机械刺激诱导成肌细胞MGF的表达上调与细胞PI的升高一致，但在3D培养中需要更强的机械力才能激活MGF的表达；(2) MGF而非IGF-IEa对机械诱导的成肌细胞复制至关重要，因为IGF-IEa的上调滞后于细胞PI的升高；(3) MGF和整合素β的时间过程上调相互关联，表明它们具有共同的机械转导途径。(C) 2009爱思唯尔公司版权所有。
Mechano-growth factor (MGF), the insulin growth factor-I (IGF-I) splice variant, has drawn an increasing attention in recent years. In this study, using a newly established system, we three-dimensionally (3D) cultured rat skeletal myoblasts and loaded them with cyclic uniaxial tensile strain of different magnitudes or time duration. After that, the cell proliferative index (PI) and mRNA expression of MGF, IGF-IEa and integrin betal were assayed. The major findings are: (1) mechanical stimulation induced MGF upregulation commensurate with cell PI elevation both in the 3D and 2D cultured myoblasts, but stronger mechanical force was needed to activate MGF expression in the 3D cultures; (2) MGF but not IGF-IEa was essential for mechano-induced myoblast replication, as IGF-IEa upregulation lagged cell PI elevation; and (3) the time course upregulation of MGF and integrin betal was correlated with each other, suggesting they shared a common mechanotransduction pathway. (C) 2009 Elsevier Inc. All rights reserved.