Genetic analysis of high α-tocopherol content in soybean seeds
Genetic analysis of high α-tocopherol content in soybean seeds
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DOI：
10.1270/jsbbs.57.23
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发表时间：
2007-03-01
期刊：
影响因子：
2.4
通讯作者：
Kitamura, Keisuke
中科院分区：
文献类型：
--
作者：
Dwiyanti, Maria Stefanie;Ujiie, Aya;Kitamura, Keisuke
生育酚是大豆中主要的亲脂性抗氧化剂，被称为维生素E的成员。由于大豆种子含有低浓度的α-生育酚（α-Toc）（具有最高维生素E活性的生育酚同种型），因此已经实施了一些育种努力以增加种子中的α-生育酚含量。在本研究中，我们使用来自具有高α-生育酚含量的品种Keszthelyi A.S.（α-Toc 20-30%）和日本栽培品种Ichihime（α-Toc < 10%）进行高α-生育酚浓度遗传分析。F-2植株（173粒种子）群体中α-生育酚和总生育酚含量之间没有显著相关性，表明α-生育酚和总生育酚含量在种子中是独立调节的。因此，可以增加α-生育酚浓度而不影响总生育酚含量。α-生育酚浓度性状的广义遗传力为0.645，表明γ-生育酚浓度性状具有较高的遗传力。卡方拟合优度检验表明，Sat_243和Sat_167标记（连锁群K）与α-生育酚浓度显著相关（P < 0.05）。这些标记可用于分子标记辅助选择高生育酚大豆品种。对F-2种子和F-2植物（173粒种子）的生育酚组成分析表明，α-生育酚和γ-生育酚浓度之间存在显著的负相关性，表明γ-生育酚甲基转移酶（将γ-生育酚转化为α-生育酚的酶）的活性在α-生育酚浓度高的个体中较高。
Tocopherols, known as members of vitamin E, are the major lipophilic antioxidants in soybean. Since soybean seeds contain a low concentration of alpha-tocopherol (alpha-Toc), the tocopherol isoform with the highest vitamin E activity, some breeding efforts have been implemented to increase the alpha-tocopherol content in the seeds. In the present study, we used F-2 seed and F-2 plant (173 seeds) populations derived from a cross between a variety with a high alpha-tocopherol content, Keszthelyi A.S. (alpha-Toc 20-30%) and a Japanese cultivar, Ichihime (alpha-Toc < 10%) for genetic analysis of the high alpha-tocopherol concentration. There was no significant correlation between the alpha-tocopherol and total tocopherol contents in the F-2 plant (173 seeds) population, suggesting that the alpha-tocopherol and total tocopherol contents were regulated independently in the seeds. Therefore, it may be possible to increase the alpha-tocopherol concentration without affecting the total tocopherol content. Broad-sense heritability value for the alpha-tocopherol concentration trait was estimated to be 0.645 in the F-2 seeds, suggesting that the gamma-tocopherol concentration trait was highly heritable. Chi-square goodness-of-fit test for evaluating the relationship between genotype of certain SSR markers and the alpha-tocopherol concentration showed that the Sat_243 and Sat_167 markers (linkage group K) were significantly associated with the alpha-tocopherol concentration (P < 0.05). These markers could be used for marker-assisted selection to breed soybean cultivars with high alpha-tocopherol concentrations. Analysis of the F-2 seeds and the F-2 plants (173 seeds) for tocopherol composition showed that there was a significantly negative correlation between the alpha-tocopherol and gamma-tocopherol concentrations, suggesting that the activity of gamma-tocopherol methyltransferase, the enzyme that converts gamma-tocopherol to alpha-tocopherol, is high in individuals with a high alpha-tocopherol concentration.