A transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein
A transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein
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跨膜结构域决定了水稻条纹病毒 pc4 向胞间连丝的定位，并且对于其作为运动蛋白的功能至关重要
DOI：
10.1016/j.virusres.2014.02.006
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发表时间：
2014-04-21
期刊：
影响因子：
5
通讯作者：
Chen, Jianping
中科院分区：
文献类型：
--
作者：
Rong, Lingling;Lu, Yuwen;Chen, Jianping
水稻条纹病毒（rice stripe virus，RSV）编码的pc 4蛋白是一种病毒运动蛋白（viral movement protein，MP）。跨膜（TM）结构域跨越AAs 106-123的pc 4被确定，并证明是必不可少的定位pc 4的胞间连丝（PD）（但不是叶绿体），并恢复运动缺陷的PVX的运动的能力。丙氨酸扫描突变体的分析表明，M116 A和G120 A具有类似的定位野生型pc 4，被定位在PD和叶绿体，但所有其他突变体只定位在叶绿体，而不是在PD。不能定位于PD的突变体几乎没有（G123 A）或没有恢复PVX-GFP Delta p25运动的能力，表明PD定位对于pc 4作为运动蛋白的功能至关重要。奇怪的是，突变体M116 A和G120 A定位于PD，并保留了结合单链RNA的能力，但不支持PVX-GFR Delta p25移动，这表明除了PD定位和核苷酸结合能力之外，pc 4作为移动蛋白的功能可能还需要其他特性。(c)2014爱思唯尔有限公司版权所有。
The pc4 protein encoded by rice stripe virus (RSV) is a viral movement protein (MP). A transmembrane (TM) domain spanning AAs 106-123 of pc4 was identified and shown to be essential for localization of pc4 to plasmodesmata (PD) (but not to chloroplasts) and for its ability to recover the movement of movement-deficient PVX. Analysis of alanine-scanning mutants showed that M116A and G120A had a similar localization to wild type pc4, being localized at PD and chloroplasts, but all other mutants were only localized at chloroplasts and not at PD. Mutants that could not be localized at PD had little (G123A) or no ability to recover PVX-GFP Delta p25 movement, indicating that PD localization is crucial for the function of pc4 as a movement protein. Strangely, mutants M116A and G120A localized at PD and retained the ability to bind single-stranded RNA but did not support PVX-GFR Delta p25 movement, indicating that properties other than PD localization and nucleotide binding ability may be needed for the function of pc4 as a movement protein. (c) 2014 Elsevier B.V. All rights reserved.