Mouse exo utero development system: Protocol and troubleshooting
Mouse exo utero development system: Protocol and troubleshooting
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DOI：
10.1111/j.1741-4520.2008.00203.x
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发表时间：
2008-12-01
影响因子：
1.3
通讯作者：
Otani, Hiroki
中科院分区：
文献类型：
--
作者：
Yamada, Makiko;Hatta, Toshihisa;Otani, Hiroki
子宫外发育系统使我们能够在妊娠中期至晚期（从器官发生晚期到组织发生期）对小鼠或大鼠的活胚胎进行操作或操作，并使它们在原位存活，直到在所需的时间点分析其影响。我们可以检查将生物活性分子或细胞注射到活胚胎的目标部位，破坏特定的胚胎区域或进行胎儿手术的效果。该系统比建立转基因小鼠系更简单，更省时，更经济，为发育科学家提供了一个微调的实验设计。为了推广使用小鼠子宫外发育系统，我们详细介绍了技术程序，讨论了系统故障的关键点，并举例说明了一些必要的仪器胎儿显微注射。
The exo utero development system allows us to manipulate or operate on live embryos of mice or rats at mid- to late gestation stages, from late organogenetic to histogenetic periods, and keep them alive in situ until the analysis of their effects at a desired time point. We can examine the effects of injecting bioactive molecules or cells into targeted parts of a live embryo, destroying specific embryonic regions, or performing fetal surgery. This system is far simpler and more time- and cost-effective for in vivo functional analyses than establishing genetically modified mouse lines and provides a fine-tuned experiemental design for developmental scientists. To promote use of the mouse exo utero development system, we elaborate on the technical procedures, discuss critical points for troubleshooting the system, and illustrate some apparatuses essential for fetal microinjection.