Class D and Bsister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.)
Class D and Bsister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.)
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DOI：
10.1007/s11103-009-9504-z
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发表时间：
2009-06
影响因子：
5.1
通讯作者：
Kaori Yamada;T. Saraike;N. Shitsukawa;C. Hirabayashi;S. Takumi;K. Murai
中科院分区：
文献类型：
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作者：
Kaori Yamada;T. Saraike;N. Shitsukawa;C. Hirabayashi;S. Takumi;K. Murai
在面包小麦(Triticum aestivumL.)细胞质替代(异质)系中，雄蕊向雌蕊的同源异型转化已有报道。它们的细胞质与一种近缘野生物种粗毛山羊草相同。异源胚珠在异质小麦的雌蕊状雄蕊中分化。SEEDSTICK(STK)样D类MADS-box基因小麦STK(WSTK)在雌蕊状雄蕊异位胚珠原基和真雌蕊中均有表达，表明异位胚珠的形成是WSTK在异质小麦雌蕊状雄蕊中表达的结果。异位胚珠不能形成完整的珠被，是不正常的。根据WSTK基因和小麦MADS-box基因WBsis的表达模式，我们得出结论：WSTK在类雌蕊中起着决定胚珠特性的作用，但由于WBsis的异常表达，导致胚珠不能完全发育。
Homeotic transformation of stamens into pistil-like structures (pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivumL.) that have the cytoplasm of a related wild species,Aegilops crassa. An ectopic ovule differentiates in the pistil-like stamen in the alloplasmic wheat. TheSEEDSTICK(STK)—like class D MADS-box gene,wheat STK(WSTK), was expressed in the primordia of ectopic ovules in the pistil-like stamens as well as in the true pistil, suggesting that ectopic ovule formation results fromWSTKexpression in the pistil-like stamens of alloplasmic wheat. The ectopic ovule is abnormal as it fails to form complete integuments. Based on the expression pattern ofWSTKand BsisterMADS-box gene,WBsis(wheat Bsister), we conclude thatWSTKplays a role in determination of ovule identity in the pistil-like stamen, but complete ovule development fails due to aberrant expression ofWBsis.