INVITRO CHARACTERIZATION OF LUNG CANCERS BY THE USE OF H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF TISSUE-EXTRACTS AND DISCRIMINANT FACTOR-ANALYSIS
INVITRO CHARACTERIZATION OF LUNG CANCERS BY THE USE OF H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF TISSUE-EXTRACTS AND DISCRIMINANT FACTOR-ANALYSIS
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DOI：
10.1002/mrm.1910290403
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发表时间：
1993-04-01
影响因子：
3.3
通讯作者：
YASUDA, N
中科院分区：
文献类型：
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作者：
HANAOKA, H;YOSHIOKA, Y;YASUDA, N
应用质子磁共振波谱（H-1 MRS）技术，对四种类型肺癌（鳞癌、腺癌、大细胞、小细胞）和正常肺组织的离体提取液进行了波谱分析。氯仿/甲醇-水提取物的亲水相产生了几个明显的峰。其中胆碱、肌酸、甘氨酸和丙氨酸的峰面积及其比值被计算并用作表征不同肺组织的参数。正常肺组织的胆碱/丙氨酸和甘氨酸/丙氨酸比值均显著高于肺癌组织（P < 0.001 ~ P < 0.05）。肌酸/甘氨酸和肌酸/胆碱通常在任何两种类型的肺癌之间提供良好的区分（P < 0.001至P < 0.05）。当数据进一步用判别因素分析进行分析时，在正常肺和每种癌症类型之间或在四种肺癌类型之间的预测准确率为81.5%至90.7%。这些结果表明，H-1 MRS可能是有用的辅助手段，在肺癌的鉴别诊断。
Using proton magnetic resonance spectroscopy (H-1 MRS) spectra were obtained in vitro from extracts of four types of lung cancer (squamous cell, adenocarcinoma, large cell, small cell) and normal lung. The hydrophilic phase of the chloroform/methanol-water extracts yielded several distinct peaks. Among them the peak areas for cholines, creatines, glycine, and alanine, and their ratios were calculated and used as parameters to characterize different lung tissues. The ratios, cholines/alanine and glycine/alanine, were significantly (P < 0.001 to P < 0.05) higher for the normal lung than lung cancers. Creatines/glycine and creatines/cholines generally provided good discrimination (P < 0.001 to P < 0.05) between any two types of lung cancer. When data were further analyzed by discriminant factor analysis, there was 81.5 to 90.7% accuracy in predicting between normal lung and each cancer type, or among the four types of lung cancer. These results suggested that H-1 MRS might be useful as an adjunct modality in the differential diagnosis of lung cancers.