Establishment of a new cell line with the characteristics of a multipotential progenitor from a patient with chronic myelogenous leukemia in early erythroblastic crisis.
Establishment of a new cell line with the characteristics of a multipotential progenitor from a patient with chronic myelogenous leukemia in early erythroblastic crisis.
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从患有早期成红细胞危象的慢性粒细胞白血病患者中建立具有多能祖细胞特征的新细胞系。
作者：
T. Furukawa;T. Koike;W. Ying;K. Kishi;S. Aoki;T. Gotoh;S. Hashimoto;H. Saitoh;M. Hanano;S. Shinada
从1例慢性粒细胞白血病急变期患者中建立了一个新的细胞系（KH 88）。白血病原始细胞具有未分化原始细胞的特征，嗜碱性无颗粒细胞质，酸性磷酸酶和α-醋酸萘酯酶呈局灶性阳性。CD 36、CD 33、HLADR和CD 71在原始细胞表面表达。大多数原始细胞血小板过氧化物酶活性呈阳性，其中一些颗粒含有铁蛋白分子的聚集体。这些发现与“早期”成红细胞白血病的发现一致，该已建立的细胞系（KH 88）具有相似的特征，并且实际上产生血红蛋白A和血红蛋白F。尽管KH 88细胞对巨核细胞标志物呈阴性，但它们被佛波酯诱导表达CD 41。此外，少数KH 88细胞对髓过氧化物酶呈阳性。该细胞系具有分化为三个谱系的能力，为研究多能干细胞的分化提供了一个有用的模型。此外，KH 88的一个亚系有一个特殊的染色体异常，del（3）（q21 q25），这将有助于研究这种染色体异常的意义。
A novel cell line (KH88) was established from a patient with chronic myelogenous leukemia in blastic crisis. The leukemic blasts had the features of undifferentiated blasts with basophilic agranular cytoplasm and they were focally positive for acid phosphatase and alpha-naphthyl acetate esterase. CD36, CD33, HLADR, and CD71 were expressed on the surfaces of the blast cells. Most blasts were positive for platelet peroxidase activity, and some of them had granules containing aggregates of ferritin molecules. These findings were compatible with those of 'early' erythroblastic leukemia, this established cell line (KH88) having similar characteristics, and actually producing hemoglobin A and hemoglobin F. Although the KH88 cells were negative for megakaryocytic markers, they were induced to express CD41 by phorbol ester. Further, a few KH88 cells were positive for myeloperoxidase. This cell line was thus revealed to have the capacity to differentiate into three lineages, providing a useful model for studying the differentiation of multipotential stem cells. Moreover, a subline of KH88 had a peculiar chromosome abnormality, del(3)(q21q25); it would be useful to study the significance of this chromosomal abnormality.