Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies.
Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies.
复制标题
纳米颗粒诱导的基因毒性研究中磷酸化组蛋白 H2AX 的测定。
DOI：
10.1007/978-1-4939-8916-4_9
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zhang,Qunwei
中科院分区：
文献类型：
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作者：
Wan,Rong;Mo,Yiqun;Tong,Ruirui;Gao,Meiqin;Zhang,Qunwei
DNA双链断裂（DNA double-strand breaks，DSB）是由各种遗传毒性损伤引发的最严重的DNA损伤之一，如果不能有效修复，可导致染色体改变、基因组不稳定甚至肿瘤发生。在DNA损伤时，组蛋白H2 AX分子在DNA DSB位点附近的丝氨酸139处迅速磷酸化，形成γ-H2 AX灶。γ-H2 AX作为一种与DNA损伤和修复相关的早期重要细胞事件，是一种高度敏感的DNA损伤“监测”生物标志物，因此在遗传毒理学筛查中具有重要的应用价值。我们和其他研究人员使用γ-H2 AX作为标记物，以评估某些纳米颗粒在体外和体内的潜在遗传毒性作用。在本章中，我们描述了几种有用的γ-H2 AX检测方法，这些方法可用于评估纳米颗粒的潜在遗传毒性效应。
DNA double-strand breaks (DSBs), one of the most severe lesions of DNA damage triggered by various genotoxic insults, can lead to chromosome change, genomic instability, and even tumorigenesis if not repaired efficiently. In response to DNA damage, histone H2AX molecules are rapidly phosphorylated at serine 139 near the site of DNA DSBs and form γ-H2AX foci. As an early important cellular event linked to DNA damage and repair, γ-H2AX is a highly sensitive biomarker for “monitoring” DNA damage and consequently is a useful tool in genetic toxicology screen. We and other researchers have used γ-H2AX as a marker to assess the potential genotoxic effects of some nanoparticles in vitro and in vivo. In this chapter, we describe several useful methods for γ-H2AX detection, which can be used to evaluate the potential genotoxic effects of nanoparticles.