Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism
Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism
复制标题
DOI：
10.1007/bf01586186
复制
发表时间：
1996-01-01
影响因子：
4.5
通讯作者：
Mooi, FR
中科院分区：
文献类型：
--
作者：
Frenay, HME;Bunschoten, AE;Mooi, FR
蛋白A基因（spa）的多态性X-区域用于耐甲氧西林金黄色葡萄球菌（MRSA）菌株的分子分型。X区域的特征是可变数字体外和体内生长的MRSA菌株的分析显示X-区对于MRSA菌株的流行病学分型足够稳定。将MRSA菌株的Spa分型与噬菌体分型进行比较，并且通常在两种方法之间发现一致性。然而，SPA分型更敏感，可以区分特定噬菌体类型内的菌株。SPA分型获得的结果表明，医院爆发可能由两种或多种MRSA菌株引起。SPA分型可能是揭示MRSA菌株在医院内和医院之间传播的重要工具。
The polymorphic X-region of the protein A gene (spa) was used for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains. The X-region is characterized by a variable number (between 3 and 15) of small repeats, DNA sequencing of MRSA strains revealed 25 distinct repeats, Analysis of MRSA strains grown in vitro and in vivo revealed that the X-region was sufficiently stable for epidemiologic typing of MRSA strains, Spa typing of MRSA strains was compared to phage typing and, in general, concordance was found between the two methods, However, spa typing was more sensitive, allowing differentiation of strains within a particular phage type, Results obtained with spa typing suggest that hospital outbreaks may be caused by two or more MRSA strains. Spa typing may be an important tool in unravelling the spread of MRSA strains within and between hospitals.