GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes
GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes
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DOI：
10.1038/sj.onc.1200898
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发表时间：
1997-02-27
期刊：
影响因子：
8
通讯作者：
Lang, JC
中科院分区：
文献类型：
--
作者：
Kenny, JJ;Knobloch, TJ;Lang, JC
我们的实验室先前描述了使用来自CML白血病患者的DNA通过NIH 3 T3转化测定法独立分离成纤维细胞生长因子4（FGF-4）基因（Lucas et al.，1994年）。FGF-4基因通过DNA重排被截短，并被命名为GRS。在这篇手稿中，我们描述了GRS cDNA的分离，并通过序列比较表明，GRS是Bcl-2基因家族的一个新成员。北方分析显示该基因在正常人体组织中的表达主要限于造血区室。癌细胞系中基因表达模式的分析表明GRS在造血系统恶性肿瘤和黑色素瘤中表达。GRS的染色体定位也已确定。该基因位于15号染色体上q24-25带内。
Our laboratory previously described the independent isolation of the fibroblast growth factor 4 (FGF-4) gene by NIH3T3 transformation assay using DNA from a patient with CML leukemia (Lucas et al., 1994). The FGF-4 gene was truncated by DNA rearrangement with a novel gene named GRS. In this manuscript we describe isolation of GRS cDNA and show by sequence comparison that GRS is a novel member of the Bcl-2 gene family. Northern analysis shows expression of the gene in normal human tissue to be largely restricted to the hematopoietic compartment. Analysis of the pattern of gene expression in cancer cell lines demonstrates GRS is expressed in hematopoietic malignancies and in melanoma. The chromosomal location of GRS has also been determined. The gene is positioned on chromosome 15 within bands q24-25.