Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats.
Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats.
复制标题
小黄帽大鼠和斯普拉格道利大鼠的血小板和大脑中的血清素摄取和丙咪嗪结合。
DOI：
10.1016/0024-3205(83)90792-0
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
Meltzer,HY
中科院分区：
文献类型：
--
作者：
Arora,RC;Tong,C;Jackman,HL;Stoff,D;Meltzer,HY
比较了具有 5-HT 储存异常的雄性 Fawn-hooded 大鼠与正常 Sprague-Dawley 大鼠的突触体和血小板的血清素 (5-HT) 摄取和丙咪嗪结合动力学参数。小黄帽大鼠突触体的 5-HT 摄取 Vmax 显着高于 Sprague-Dawley 大鼠，而血小板的 Vmax 没有差异。两种大鼠品系的突触体和血小板的Km值均无显着差异。 Fawn-hooded大鼠的血小板和脑上的 3 H-丙咪嗪结合的Bmax值显着低于Sprague-Dawley大鼠。 Fawn-hooded 大鼠与血小板膜结合的丙咪嗪 Kdof 显着低于 Sprague-Dawley 大鼠，但突触体的 Kdof 没有显着差异。这些结果表明 5-HT 摄取和丙咪嗪结合位点可能是独立调节的，并且血小板并不总是表现出大脑 5-HT 神经元中存在的异常，反之亦然。
The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmaxfor 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The Kmvalues of both the synaptosomes and platelets of the two rat strains were not significantly different. The Bmaxvalues for3H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The Kdof imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the Kdof the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.