Intracellular electrolytes in dystrophic mouse muscles
Intracellular electrolytes in dystrophic mouse muscles
复制标题
营养不良小鼠肌肉的细胞内电解质
DOI：
10.1016/0014-4886(72)90105-7
复制
发表时间：
1972
影响因子：
5.3
通讯作者：
B. Salafsky
中科院分区：
文献类型：
--
作者：
J. Hoh;B. Salafsky
肌营养不良症是一组影响人类和几种动物的骨骼肌遗传性疾病。营养不良肌肉比正常肌肉单位重量含有更多的Na+和更少的K+(2,8,15)。电解质含量的差异至少部分是由于肌肉组织的损失和结缔组织的替代，导致纤维内空间减少，纤维外空间增加(9)。细胞内电解质水平的变化是否会导致营养不良肌肉中Na+和Ii+含量的变化？这个问题很重要，因为我们最近通过神经交叉结合实验表明，大鼠肌肉中的细胞内电解质受神经调节(7)。如果答案是肯定的，就会提出一种有趣的可能性，即神经对肌肉电解质的调节影响可能在肌肉萎缩症中存在缺陷。营养不良肌纤维与正常肌纤维的电生理特性存在差异，提示细胞内电解质组成可能存在差异。文献中普遍认为（4,6,9,10,12,13），尽管从不同实验室获得的值有很大差异，但观察到的营养不良小鼠肌纤维的静息膜电位明显低于正常肌纤维，这可能是因为所使用肌肉的纤维类型组成和采样技术的差异。通过在老鼠腓肠肌的整个厚度取样纤维，克里曼。Patridge和Glaser(9)发现正常纤维的平均值为92.5 mv，营养不良纤维的平均值为84.9 mv。人类营养不良的肌肉纤维
Muscular dystrophy is a group of hereditary diseases of skeletal muscle affecting man and several species of animals. Dystrophic muscles contain more Na+ and less K+ per unit weight than normal muscles (2, 8, 15). This difference in electrolyte content is due, at least in part, to loss of muscle tissue and its replacement by connective tissue, resulting in a decrease in intrafiber space with an increase in extrafiber space (9). Do changes in intracellular electrolyte levels contribute to changes in Na+ and Ii+ content of dystrophic muscles? This question is important because we have recently shown by nerve cross-union experiments that intracellular electrolytes in rat muscles are under neural regulation (7). An affirmative answer would suggest the interesting possibility that the neural regulatory influence for muscle electrolytes may be defective in muscular dystrophy. There are differences in electrophysiological properties of dystrophic and normal muscle fibers which suggest that there may be differences in intracellular electrolyte composition. There is general agreement in the literature (4, 6, 9, 10, 12, 13) that the resting membrane potential of dystrophic mouse muscle fibers measured in view are significantly lower than those of normal muscle fibers, though values obtained from different laboratories differ significantly, presumably because of differences in fiber type composition of muscles used and in sampling techniques. By sampling fibers through the entire thickness of the gastrocnemius muscle of the mouse, Kleeman. Patridge and Glaser (9) found a mean value of 92.5 mv for normal and 84.9 mv for dystrophic fibers. Human dystrophic muscle fibers