Ecdysteroid-regulated heat-shock gene expression during Drosophila melanogaster development.
Ecdysteroid-regulated heat-shock gene expression during Drosophila melanogaster development.
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DOI：
10.1016/0012-1606(86)90263-0
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发表时间：
1986-06
影响因子：
2.7
通讯作者：
S. Thomas;J. Lengyel
中科院分区：
文献类型：
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作者：
S. Thomas;J. Lengyel
HSP26、28和83RNA水平的峰值与胚胎发育中期、蛹和中期蜕皮类固醇滴度的峰值相关，并且与74EF蜕皮激素羽化时的RNA水平峰值相关。条件性蜕皮激素缺乏菌株ecinecd-1的温移抑制了蜕皮类固醇的峰值，随后HSP 26 RNA消失，HSP 83 RNA水平下降；随后向温移菌株中加入外源20-OH-蜕皮激素，导致HSP 83 RNA水平增加几倍，HSP 26 RNA水平急剧增加。这些结果与蜕皮类固醇在几个发育阶段对HSP83、28和26基因的诱导是一致的。
Peaks in hsp 26, 28, and 83 RNA levels are correlated with peaks in ecdysteroid titers during mid-embryogenesis, pupariation, and mid-pupation, and with a peak in the level of RNA from the 74EF ecdysone puff at pupariation. Inhibition of the ecdysteroid peak at pupariation by temperature shift of the conditionally ecdysteroid-deficient strainecd-1was followed by a disappearance of hsp 26 RNA and a decline in hsp 83 RNA level; subsequent addition of exogeneous 20-OH-ecdysone to the temperature-shifted strain resulted in a severalfold increase in hsp 83 RNA level, and a dramatic increase in that of hsp 26. These results are consistent with the induction of the hsp 83, 28, and 26 genes by ecdysteroid at several developmental stages.