EFFECT OF AGE ON NADPH-DIAPHORASE-CONTAINING MYENTERIC NEURONS OF RAT ILEUM AND PROXIMAL COLON
EFFECT OF AGE ON NADPH-DIAPHORASE-CONTAINING MYENTERIC NEURONS OF RAT ILEUM AND PROXIMAL COLON
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DOI：
10.1007/bf00318495
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发表时间：
1995-02-01
影响因子：
3.6
通讯作者：
BURNSTOCK, G
中科院分区：
文献类型：
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作者：
BELAI, A;COOPER, S;BURNSTOCK, G
为探讨胎龄对NADPH-黄递酶阳性神经元在胚胎第19天大鼠回肠肌间神经丛和近端结肠内分布的影响，以及出生后第4、6和26月龄大鼠回肠肌间神经丛内NADPH-黄递酶神经元的分布，我们观察了胎龄对NADPH-黄递酶神经元分布的影响。每个神经节NADPH黄递酶染色的神经元的平均百分比是使用蛋白基因产物9.5(蛋白质存在于大多数神经元中，如果不是全部神经元中的蛋白质)免疫染色的神经元为100%。结果表明，26月龄大鼠近端结肠肌间神经丛中NADPH-黄递酶阳性神经元随增龄而明显增多，NADPH-黄递酶免疫反应阳性神经元几乎全部为蛋白基因产物9.5。这与回肠形成鲜明对比，在回肠，NADPH-黄递酶阳性神经元在老年大鼠中没有明显的相对增加。讨论了这些发现与程序性细胞存活和细胞死亡有关的意义。
The effect of age on the distribution of NADPH-diaphorase-containing neurones was investigated in the myenteric plexus of ileum and proximal colon of embryonic day-19 rats, as well as in rats at postnatal day 4, 6 months and 26 months. The mean percentage of NADPH-diaphorase-stained neurones per ganglion was established using protein gene product 9.5(protein found in most if not all neurones)-immunostained neurones as 100%. The results revealed that there was a significant relative increase in NADPH-diaphorase-positive neurones with increasing age in the myenteric plexus of proximal colon with nearly all protein gene product 9.5-immunoreactive neurones staining for NADPH diaphorase in 26-month-old rats. This was in marked contrast with the ileum, where no significant relative increase in NADPH-diaphorase-positive neurones was seen in aged rats. The implications of these findings in relation to programmed cell survival and cell death are discussed.