Characterization of Critical Functions of Long Non-Coding RNAs and mRNAs in Rhabdomyosarcoma Cells and Mouse Skeletal Muscle Infected by Enterovirus 71 Using RNA-Seq.
Characterization of Critical Functions of Long Non-Coding RNAs and mRNAs in Rhabdomyosarcoma Cells and Mouse Skeletal Muscle Infected by Enterovirus 71 Using RNA-Seq.
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使用 RNA-Seq 表征肠道病毒 71 感染的横纹肌肉瘤细胞和小鼠骨骼肌中长非编码 RNA 和 mRNA 的关键功能
DOI：
10.3390/v10100556
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发表时间：
2018-10-11
期刊：
影响因子：
--
通讯作者：
Duan G
中科院分区：
文献类型：
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作者：
Li Y;Zhang C;Qin L;Li D;Zhou G;Dang D;Chen S;Sun T;Zhang R;Wu W;Xi Y;Jin Y;Duan G
肠道病毒71型（EV 71）是重症手足口病（HFMD）的主要病原体。长链非编码RNA（lncRNA）被认为是病毒感染发病机制中的关键因素。然而，lncRNA在EV 71-宿主相互作用中的关键功能尚未被表征。在这里，我们第一次使用第二代测序对EV 71感染的人横纹肌肉瘤（RD）细胞和小鼠骨骼肌中的lncRNA和mRNA表达谱进行了全局转录组分析。在我们的研究中，总共鉴定了3801个新的lncRNA。此外，23个lncRNA和372个mRNA在感染和未感染的RD细胞之间的表达水平存在显著差异，而104个lncRNA和2647个mRNA在感染的新生小鼠骨骼肌中差异表达。综合生物信息学分析包括靶基因预测、lncRNA-mRNA共表达网络构建、基因本体（GO）和京都基因与基因组百科全书（KEGG）途径分析，主要针对差异表达基因（DEG）。我们的研究结果表明，lncRNA可能通过调节免疫反应，蛋白质结合，细胞成分的生物合成和代谢参与EV 71感染诱导的发病机制。本研究为进一步了解lncRNA的功能和EV 71感染后可能的致病机制提供了新的视角。
Enterovirus 71 (EV71) is the main pathogen of severe hand-foot-mouth disease (HFMD). Long non-coding RNAs (lncRNAs) are recognized as pivotal factors during the pathogenesis of viral infection. However, the critical functions of lncRNAs in EV71–host interactions have not been characterized. Here, for the first time, we performed global transcriptome analysis of lncRNA and mRNA expression profiles in EV71-infected human rhabdomyosarcoma (RD) cells and skeletal muscle of mice using second-generation sequencing. In our study, a total of 3801 novel lncRNAs were identified. In addition, 23 lncRNAs and 372 mRNAs exhibited remarkable differences in expression levels between infected and uninfected RD cells, while 104 lncRNAs and 2647 mRNAs were differentially expressed in infected skeletal muscle from neonatal mice. Comprehensive bioinformatics analysis included target gene prediction, lncRNA-mRNA co-expression network construction, as well as gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis mainly focused on differentially-expressed genes (DEGs). Our results suggest that lncRNAs may participate in EV71 infection-induced pathogenesis through regulating immune responses, protein binding, cellular component biogenesis and metabolism. The present study provides novel insights into the functions of lncRNAs and the possible pathogenic mechanism following EV71 infection.
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DOI：
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发表时间：
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