Phenotypic and genotypic characterization of Vibrio vulnificus: Proposal for the substitution of the subspecific taxon biotype for serovar
Phenotypic and genotypic characterization of Vibrio vulnificus: Proposal for the substitution of the subspecific taxon biotype for serovar
复制标题
DOI：
10.1128/aem.63.4.1460-1466.1997
复制
发表时间：
1997-04-01
影响因子：
4.4
通讯作者：
Pedersen, K
中科院分区：
文献类型：
--
作者：
Biosca, EG;Amaro, C;Pedersen, K
创伤弧菌根据表型特性和鳗鱼毒力将菌株分为两个生物型。生物型2对鳗鱼是毒力的，对吲哚反应阴性，血清学上是同质的(血清组E)，而生物型1的菌株是无毒的，吲哚阳性的，血清学上是异质性的。在本研究中，我们对主要从北欧回收的21株创伤弧菌进行了表型和基因特征分析，将它们与两种生物型的参考菌株进行比较，以寻找生物型2的新菌株。这项工作的结果表明，大多数对鳗鱼毒力强的菌株表现出以前被认为具有生物型2和HindIII的特定核型特征的表型特征。然而，在新分离的菌株中，我们发现(I)一株E群毒株对鳗鱼致病，但吲哚阳性；(Ii)一株不属于E群但对鳗鱼致病的菌株。由于没有生化测试或特定的血清群可以确定与鳗鱼的毒力有关，我们建议将创伤弧菌菌株划分为血清型而不是生物型。因此，我们建议将E血清型作为以前被归类为生物型2的菌株的命名。最后，在挪威和瑞典养殖的鳗鱼中以及在人类感染和虾中出现了E血清组。
The classification of Vibrio vulnificus strains into two biotypes has been maintained on the basis of phenotypic properties and eel virulence. Biotype 2 is virulent for eels, negative for the indole reaction, and serologically homogeneous (serogroup E), whereas strains of biotype 1 are avirulent, indole positive, and serologically heterogeneous. In the present study, we phenotypically and genotypically characterized 21 V. vulnificus isolates, recovered mainly from northern Europe, by comparing them with reference strains of both biotypes to look for new isolates of biotype 2. The results of this work revealed that the majority of isolates virulent for eels presented phenotypic traits previously considered characteristics of biotype 2 and specific ribotypes with HindIII. However, among the new isolates we found (i) a serogroup E strain virulent for eels but indole positive and (ii) one isolate not belonging to serogroup E but pathogenic for eels. Since no biochemical test or specific serogroup can with certainty be associated with eel virulence, we propose to classify V. vulnificus strains into serovars instead of biotypes. Thus, we suggest serovar E as the denomination of those strains previously classified as biotype 2, Finally, the occurrence of serogroup E in eels cultured in Norway and Sweden, as well as from human infections and shrimp, has been demonstrated.