Nonimmunochemical quantitation of mammalian apolipoprotein A-I in whole serum or plasma by nonreducing gel electrophoresis.
Nonimmunochemical quantitation of mammalian apolipoprotein A-I in whole serum or plasma by nonreducing gel electrophoresis.
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发表时间：
1989-12
影响因子：
6.5
通讯作者：
D. France;T. Hughes;R. Miserendino;J. A. Spirito;J. Babiak;J. B. Eskesen;C. Tapparelli;J. Paterniti
中科院分区：
文献类型：
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作者：
D. France;T. Hughes;R. Miserendino;J. A. Spirito;J. Babiak;J. B. Eskesen;C. Tapparelli;J. Paterniti
建立了一种快速、简便的哺乳动物apoA-I定量方法。该方法包括非还原性十二烷基硫酸钠凝胶电泳和考马斯亮蓝染色，并利用全血清或血浆中apoA-I的相对丰度。ApoA-I的分离度足以通过激光密度测定法或分光光度法进行定量。该测定在0.25至4.0 μ g apoA-I范围内呈线性。分析回收率为98%。试验内变异性为3.1%，试验间变异性为7.5%。与人apoA-I放射免疫测定法呈高度正相关（r = 0.98）。对于几个物种的调查，载脂蛋白A-I的值与高密度脂蛋白胆固醇值密切相关，呈正相关。当应用于蒙古沙鼠营养扰动的研究时，该方法检测到血清apoA-I的敏感和显着的变化，其抑制HDL胆固醇的变化。
A rapid and convenient method for the quantitation of mammalian apoA-I has been developed. The method involves nonreducing sodium dodecyl sulfate gel electrophoresis and Coomassie blue staining, and takes advantage of the relative abundance of apoA-I in whole serum or plasma. ApoA-I was sufficiently resolved to allow quantitation by laser densitometry or spectrophotometry. The assay was linear from 0.25 to 4.0 micrograms of apoA-I. Analytic recovery was 98%. Within-assay variability was 3.1% and between-assay variability was 7.5%. A high degree of positive correlative (r = 0.98) was observed with a human apoA-I radioimmunoassay. For several species investigated, the apoA-I values obtained correlated strongly and positively with high density lipoprotein cholesterol values. When applied to a study of nutritional perturbation in the Mongolian gerbil, the method detected sensible and significant changes in serum apoA-I that paralleled changes in HDL cholesterol.