DIFFERENTIATION OF 3T3-L1 FIBROBLASTS TO ADIPOCYTES INDUCED BY TRANSFECTION OF RAS ONCOGENES
DIFFERENTIATION OF 3T3-L1 FIBROBLASTS TO ADIPOCYTES INDUCED BY TRANSFECTION OF RAS ONCOGENES
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DOI：
10.1126/science.1857988
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发表时间：
1991-08-02
期刊：
影响因子：
56.9
通讯作者：
SANTOS, E
中科院分区：
文献类型：
--
作者：
BENITO, M;PORRAS, A;SANTOS, E
哺乳动物 3T3-L1 细胞在连续暴露于药理学剂量的胰岛素或生理剂量的胰岛素样生长因子 I (IGF-1) 后分化为脂肪细胞。在没有外部添加胰岛素或IGF-I的情况下，转染的ras癌基因的表达导致这些细胞分化为脂肪细胞。转染正常ras基因或酪氨酸激酶trk癌基因的细胞不会分化。用显性抑制性ras突变体转染导致分化受到抑制。将未转染的 3T3-L1 细胞暴露于胰岛素会刺激活性 Ras.GTP 复合物的形成。这些观察结果表明 Ras 蛋白参与这些细胞中由胰岛素和 IGF-I 启动的信号转导途径。
Mammalian 3T3-L1 cells differentiate into adipocytes after continuous exposure to pharmacological doses of insulin or physiological doses of insulin-like growth factor I (IGF-1). Expression of transfected ras oncogenes led to differentiation of these cells into adipocytes in the absence of externally added insulin or IGF-I. Cells transfected with normal ras genes or the tyrosine kinase trk oncogene did not differentiate. Transfection with a dominant inhibitory ras mutant resulted in inhibition of differentiation. Exposure of untransfected 3T3-L1 cells to insulin stimulated formation of the active Ras.GTP complex. These observations indicate that Ras proteins participate in signal transduction pathways initiated by insulin and IGF-I in these cells.