Insulin stimulation of iodothyronine 5'-deiodinase in rat brown adipocytes.
Insulin stimulation of iodothyronine 5'-deiodinase in rat brown adipocytes.
复制标题
胰岛素刺激大鼠棕色脂肪细胞中碘甲状腺原氨酸 5-脱碘酶。
DOI：
10.1016/0006-291x(87)90632-2
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Larsen,PR
中科院分区：
文献类型：
--
作者：
Mills,I;Barge,RM;Silva,JE;Larsen,PR
胰岛素（100-3333 μU/ml）对分散的大鼠棕色脂肪细胞的碘甲腺原氨酸5′-脱碘酶有3 - 4倍的刺激作用。脱碘酶活性从1至4小时稳定增加。胰岛素通过增加Vmax增加酶活性，而Km保持不变。从培养基中省略葡萄糖不影响胰岛素反应。对α-鹅膏蕈碱的研究表明，脱碘酶活性的增加并不是由于转录速率的增加。胰岛素的作用与α1-儿茶酚胺的作用不具有加和性，提示这两种刺激剂可能有一个或多个共同的成分。
Insulin (100–3333 μU/ml) stimulates iodothyronine 5′-deiodinase 3 to 4 fold in dispersed rat brown adipocytes. Deiodinase activity increased steadily from 1 to 4 hours. Insulin increased enzyme activity via an increase in the Vmax while the Km remained unchanged. Omission of glucose from the medium did not affect the insulin response. Studies with α-amanitin suggested that the increase in deiodinase activity was not due to an increase in the rate of transcription. The insulin effect was not additive to that of α1-catecholamines, suggesting the two stimulators might have one or more common elements.