Periodic Lamellae-Based Nanofibers for Precise Immunomodulation to Treat Inflammatory Bone Loss in Periodontitis
Periodic Lamellae-Based Nanofibers for Precise Immunomodulation to Treat Inflammatory Bone Loss in Periodontitis
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DOI：
10.1002/adhm.202303549
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发表时间：
2024-05-17
影响因子：
10
通讯作者：
Luo，En
中科院分区：
文献类型：
--
作者：
He，Ze;Liu，Yao;Luo，En
牙周炎是一种常见的伴有炎性骨丢失的口腔疾病。牙周炎的病理特征通常伴随牙周免疫微环境的失衡，导致骨再生困难。因此，需要有效的治疗策略来调节免疫环境以治疗牙周炎。在这里，通过表面定向外延结晶开发了高度取向的周期性层状聚（ε-己内酯）电纺纳米纤维（PLN）。体外实验结果表明，PLN可以精确地调节巨噬细胞向M2表型的极化。PLN极化的巨噬细胞能显著促进骨髓基质细胞的迁移和成骨分化。值得注意的是，结果表明，由PLN呈现的地形线索可以通过激活雅普来调节巨噬细胞极化，这会抑制NF-κB信号传导途径。体内实验结果表明，PLN可以抑制牙周炎性骨丢失，促进牙周炎性骨再生。作者的研究结果表明，具有周期性层状结构的地形纳米纤维是调节免疫环境以治疗牙周炎中的炎性骨丢失的有希望的策略。
Periodontitis is a common oral disease accompanied by inflammatory bone loss. The pathological characteristics of periodontitis usually accompany an imbalance in the periodontal immune microenvironment, leading to difficulty in bone regeneration. Therefore, effective treatment strategies are needed to modulate the immune environment in order to treat periodontitis. Here, highly‐oriented periodic lamellae poly(ε‐caprolactone) electrospun nanofibers (PLN) are developed by surface‐directed epitaxial crystallization. The in vitro result shows that the PLN can precisely modulate macrophage polarization toward the M2 phenotype. Macrophages polarized by PLN significantly enhance the migration and osteogenic differentiation of Bone marrow stromal cells. Notably, results suggest that the topographical cues presented by PLN can modulate macrophage polarization by activating YAP, which reciprocally inhibits the NF‐κB signaling pathway. The in vivo results indicate that PLN can inhibit inflammatory bone loss and facilitate bone regeneration in periodontitis. The authors’ findings suggest that topographical nanofibers with periodic lamellae is a promising strategy for modulating immune environment to treat inflammatory bone loss in periodontitis.