A synthetic HIS4 regulatory element confers general amino acid control on the cytochrome c gene (CYC1) of yeast.
A synthetic HIS4 regulatory element confers general amino acid control on the cytochrome c gene (CYC1) of yeast.
复制标题
合成的 HIS4 调节元件赋予酵母细胞色素 c 基因 (CYC1) 一般氨基酸控制。
DOI：
10.1073/pnas.82.2.498
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发表时间：
1985
影响因子：
11.1
通讯作者：
Fink,GR
中科院分区：
文献类型：
--
作者：
Hinnebusch,AG;Lucchini,G;Fink,GR
由酵母HIS4启动子上游序列和酵母CYC1启动子下游元件构建的杂交启动子在一般氨基酸控制下表达iso1 -细胞色素c (CYC1)，这是典型的HIS4。赋予调控的HIS4片段包含至少一个序列T-G-A-C-T-C的拷贝，该序列在HIS4和其他受一般控制的基因中重复。一个14碱基对的合成寡核苷酸含有HIS4重复序列的一个拷贝，将CYC1置于一般控制之下。该寡核苷酸的两个拷贝产生的低表达水平几乎相当于我们研究的最大的HIS4 5‘非编码片段所带来的表达水平，并直接调节一组具有CYC1启动子典型的5’端转录本的表达。比较短的合成重复序列和较大的HIS4 5’片段所带来的表达水平，揭示了在抑制生长条件下维持有效基因表达所需的额外启动子元件。
Hybrid promoters constructed from upstream sequences of the yeast HIS4 promoter and the downstream element of the yeast CYC1 promoter place iso-1-cytochrome c (CYC1) expression under the general amino acid control, typical of HIS4. HIS4 fragments that confer regulation contain at least one copy of the sequence T-G-A-C-T-C that is repeated at HIS4 and other genes subject to the general control. A 14-base-pair synthetic oligonucleotide containing a single copy of the HIS4 repeat places CYC1 under the general control. Two copies of this oligonucleotide produce a derepressed level of expression nearly equivalent to that conferred by the largest HIS4 5' noncoding fragments we examined and direct regulated expression of a set of transcripts with 5' ends typical of the CYC1 promoter. Comparison of the expression levels conferred by the short synthetic repeat and larger HIS4 5' fragments reveals additional promoter elements required for maintaining efficient gene expression under repressing growth conditions.