Synthesis of multifunctional fluorescent magnetic nanoparticles for the detection of Alicyclobacillus spp. in apple juice
Synthesis of multifunctional fluorescent magnetic nanoparticles for the detection of Alicyclobacillus spp. in apple juice
复制标题
用于检测脂环酸杆菌属的多功能荧光磁性纳米粒子的合成。
DOI：
10.1016/j.foodres.2018.07.065
复制
发表时间：
2018
影响因子：
8.1
通讯作者：
Yue Tianli
中科院分区：
文献类型：
--
作者：
Wang Zhouli;Li Xueke;Zhao Yuejuan;Yuan Yahong;Cai Rui;Yue Tianli
提出了一种多功能荧光磁性纳米颗粒同时富集和鉴定苹果汁中酸环杆菌的方法。采用溶胶-凝胶法对制备的fe3o4磁性粒子（MNPs）进行修饰，形成硅壳以提高反应性，然后通过硫醇化学将所得MNPs@SiO2-SH纳米粒子与巯基乙酸功能化的CdTe/CdS量子点偶联。表征评价结果表明，MNPs-QD纳米复合材料具有良好的磁性能和光学表征。通过酯化反应将抗alicybacillusigg抗体固定在MNPs-QD材料表面。抗体的最大固定量为119.62 μg/mg，吸附反应可在60 min内完成。吸附过程可分别用Langmuir模型和拟二级动力学方程表示。mnps - qds抗体结合物具有较高的免疫捕获效率和灵敏的荧光特性，可用于绿藻杆菌污染的识别。并建立了靶细胞检测的定量分析方法。最低定量限为104CFU（菌落形成单位）/mL， 90 min即可完成检测过程。结果表明，mnps - qds抗体偶联物可成功应用于藻环杆菌的免疫捕获和检测。在苹果汁里。也就是说，所开发的mnps - qds抗体偶联物在靶菌的免疫捕获和识别方面表现出了更大的吸引力和潜力，充分展示了一种富集和快速检测藻环杆菌的新方法。在果汁里。
An approach based on multifunctional fluorescent magnetic nanoparticles was proposed for the enrichment and identification of Alicyclobacillus spp. in apple juice simultaneously. The prepared Fe3O4magnetic particles (MNPs) were modified by the sol-gel process and a silica shell was formed to improve the reactivity, and then the obtained MNPs@SiO2-SH nanoparticles were conjugated with Thioglycolic acid functionalized CdTe/CdS QDs via thiols chemistry. The characteristic evaluation results indicated that the MNPs-QD nanocomposites exhibited good magnetic properties and optical characterization. The polyclonal anti-AlicyclobacillusIgG antibody was immobilized onto the surface of MNPs-QD materials via esterification reactions. The maximum antibody immobilization capacity was 119.62 μg/mg and the adsorption reaction could be accomplished in 60 min. The adsorption process could be represented by Langmuir model and pseudo-second order kinetics equation, respectively. Based on the high immunocapture efficiency and sensitive fluorescence characteristics, the obtained MNPs-QDs-antibody conjugates could be applied to recognize the contamination ofAlicyclobacillusspp. and a quantitative analysis method was established for target cells detection. The minimum quantitative limit was 104CFU (colony forming unit)/mL and the testing process could be completed in 90 min. The results indicated that the MNPs-QDs-antibody conjugates can be successfully applied for immunocapture and detection ofAlicyclobacillusspp. in apple juice. That is to say, the developed MNPs-QDs-antibody conjugates have exhibit more attractive and great potential for the immunocapture and recognition of target bacteria, fully demonstrated a new method for enrichment and rapid detection ofAlicyclobacillusspp. in fruit juices.