Biomaterials for pluripotent stem cell engineering: From fate determination to vascularization.
Biomaterials for pluripotent stem cell engineering: From fate determination to vascularization.
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DOI：
10.1039/c5tb02658j
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发表时间：
2016-05-28
期刊：
影响因子：
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通讯作者：
Varghese S
中科院分区：
文献类型：
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作者：
Seale NM;Varghese S
材料科学和工程的最新进展可能是克服目前阻碍干细胞疗法临床转化的可重复性和可扩展性限制的关键。干细胞的生物材料辅助分化承诺及其体内功能的调节可能对以干细胞为中心的再生医学方法和下一代技术平台产生重大影响。合成生物材料特别令人感兴趣，因为它们提供了一种一致的、化学定义的和可调的方式来模拟天然组织或细胞环境的物理和化学性质。结合新兴的生物材料和生物纤维的进步，最终可能会给研究人员提供工具来调节时空复杂性，并设计出层次更复杂、生理相关的组织模拟物。在这篇综述中，我们重点介绍了生物材料辅助多能干细胞（PSC）扩增和三维（3D）组织形成策略的最新研究进展。此外，由于血管化是一个重大的挑战，影响在体内功能的工程组织，我们讨论了最近的事态发展，血管化战略和评估他们的能力，以产生灌注和功能的血管系统，可以与宿主组织整合。
Recent advancements in material science and engineering may hold the key to overcoming reproducibility and scalability limitations currently hindering the clinical translation of stem cell therapies. Biomaterial assisted differentiation commitment of stem cells and modulation of their in vivo function could have significant impact in stem cell-centred regenerative medicine approaches and next gen technological platforms. Synthetic biomaterials are of particular interest as they provide a consistent, chemically defined, and tunable way of mimicking the physical and chemical properties of the natural tissue or cell environment. Combining emerging biomaterial and biofabrication advancements may finally give researchers the tools to modulate spatiotemporal complexity and engineer more hierarchically complex, physiologically relevant tissue mimics. In this review we highlight recent research advancements in biomaterial assisted pluripotent stem cell (PSC) expansion and three dimensional (3D) tissue formation strategies. Furthermore, since vascularization is a major challenge affecting the in vivo function of engineered tissues, we discuss recent developments in vascularization strategies and assess their ability to produce perfusable and functional vasculature that can be integrated with the host tissue.