OsPKS2 is required for rice male fertility by participating in pollen wall formation.
OsPKS2 is required for rice male fertility by participating in pollen wall formation.
复制标题
OsPKS2 通过参与花粉壁的形成，是水稻雄性生育力所必需的。
DOI：
10.1007/s00299-018-2265-x
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发表时间：
2018
影响因子：
6.2
通讯作者：
Li Shuangcheng
中科院分区：
文献类型：
--
作者：
Zou Ting;Liu Mingxing;Xiao Qiao;Wang Tao;Chen Dan;Luo Tao;Yuan Guoqiang;Li Qiao;Zhu Jun;Liang Yueyang;Deng Qiming;Wang Shiquan;Zheng Aiping;Wang Lingxia;Li Ping;Li Shuangcheng
OsPKS 2是拟南芥PKSB/LAP 5的水稻直向同源基因，编码一种与水稻花粉壁形成有关的聚酮合酶，在开花植物中，花粉壁保护雄配子免受各种环境胁迫和病原菌的攻击，并促进花粉萌发。绒毡层细胞中孢粉素的生物合成是花粉壁形成的关键。近年来，对拟南芥花粉壁发育过程中孢粉素代谢的研究取得了一定进展。然而，对水稻花粉壁形成过程中孢粉素合成的分子机制知之甚少。在这项研究中，我们确定了OsPKS 2，植物特异性III型聚酮合酶基因的点突变，通过影响花粉壁形成的正常进程导致水稻雄性不育。使用CRISPR/Cas9系统产生了OsPKS 2的另外两个等位基因突变体，并且也是完全雄性不育的。这一结果进一步证实了OsPKS 2控制水稻雄性育性。OsPKS 2是拟南芥PKSB/LAP 5的一个正向表达基因，具有绒毡层特异性表达模式。此外，其产物定位于内质网。结果表明，OsPKS 2基因在拟南芥花粉壁形成过程中起重要作用，并在花粉素合成中起着保守但分化的作用。
Key messageOsPKS2, the rice orthologous gene ofArabidopsis PKSB/LAP5, encodes a polyketide synthase that is involved in pollen wall formation in rice.AbstractIn flowering plants, the pollen wall protects male gametes from various environmental stresses and pathogen attacks, as well as promotes pollen germination. The biosynthesis of sporopollenin in tapetal cell is critical for pollen wall formation. Recently, progress has been made in understanding sporopollenin metabolism during pollen wall development inArabidopsis. However, little is known about the molecular mechanism that underlies the sporopollenin synthesis in pollen wall formation in rice (Oryza sativa). In this study, we identified that a point mutation inOsPKS2, a plant-specific type III polyketide synthase gene, caused male sterility in rice by affecting the normal progress of pollen wall formation. Two other allelic mutants ofOsPKS2were generated using the CRISPR/Cas9 system and are also completely male sterile. This result thus further confirmed thatOsPKS2controls rice male fertility. We also showed thatOsPKS2is an orthologous gene ofArabidopsis PKSB/LAP5and has a tapetum-specific expression pattern. In addition, its product localizes in the endoplasmic reticulum. Results suggested that OsPKS2 is critical for pollen wall formation, and plays a conserved but differentiated role in sporopollenin biosynthesis fromArabidopsis.