HTLV x-gene product: requirement for the env methionine initiation codon.
HTLV x-gene product: requirement for the env methionine initiation codon.
复制标题
HTLV x-gene 产品：需要 env 蛋氨酸起始密码子。
DOI：
10.1126/science.2990032
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Chen,IS
中科院分区：
文献类型：
--
作者：
Wachsman,W;Golde,DW;Temple,PA;Orr,EC;Clark,SC;Chen,IS
人类T细胞白血病病毒（human T-cell leukemia viruses，HTLV）是一种具有复制能力的逆转录病毒，其基因组中含有ag、pol和denv基因以及第四个基因x，该基因被认为是HTLV的转化基因。现在显示x基因的产物由至少两个内含子剪接产生的2.1-腺苷酸酶信使RNA编码。通过对该RNA进行S1核酸酶作图和对互补DNA克隆进行核酸序列分析，确定了x基因产物的完整一级结构。它是由含有env起始密码子和下一个密码子的一个核苷酸的序列编码的，该密码子剪接到HTLV-Ⅰ和HTLV-Ⅱ x基因的主要开放阅读框。
The human T-cell leukemia viruses (HTLV) are replication-competent retroviruses whose genomes containgag,pol, andenvgenes as well as a fourth gene, termed x, which is believed to be the transforming gene of HTLV. The product of the x gene is now shown to be encoded by a 2.1-kilobase messenger RNA derived by splicing of at least two introns. By means of S1 nuclease mapping of this RNA and nucleic acid sequence analysis of a complementary DNA clone, the complete primary structure of the x-gene product has been determined. It is encoded by sequences containing theenvinitiation codon and one nucleotide of the next codon spliced to the major open reading frame of the HTLV-I and HTLV-II x gene.