Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).
Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).
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新型人细胞毒性淋巴细胞特异性丝氨酸蛋白酶 cDNA 克隆 (CSP-C) 的表征。
DOI：
10.1111/j.1399-0039.1990.tb01787.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Dupont,B
中科院分区：
文献类型：
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作者：
Klein,JL;Selvakumar,A;Trapani,JA;Dupont,B
编码一种新型丝氨酸蛋白酶细胞毒性丝氨酸蛋白酶-C（CSP-C）的人cDNA克隆已从一个cDNA文库中分离出来，该文库是从一名大颗粒淋巴增生性疾病患者的重组白细胞介素-2（IL-2）激活淋巴细胞制备的。该克隆具有741个碱基对的开放阅读框，编码推定的246个氨基酸蛋白。该蛋白质序列含有丝氨酸蛋白酶的催化电荷中继系统特征和在小鼠和人中发现的成熟细胞毒性淋巴细胞丝氨酸蛋白酶的保守N末端氨基酸序列。CSP-C的氨基酸序列与先前报道的细胞毒性丝氨酸蛋白酶-B（CSP-B）/人淋巴细胞蛋白酶（HLP）/SECT具有71%的同一性，与粒细胞特异性丝氨酸蛋白酶组织蛋白酶G具有57%的同一性。与另一种淋巴细胞特异性丝氨酸蛋白酶人光明节因子（HF）/粒酶A的同源性为41%。转录本在用IL-2或IL-2加植物血凝素（PHA）刺激的淋巴细胞中表达。CSP-C在B淋巴母细胞样细胞系或T白血病细胞系MOLT 4中不表达。cDNA序列表明，该蛋白表达为前原肽，如已发现在其他小鼠和人类淋巴细胞来源的丝氨酸蛋白酶。最近有报道称，人类染色体14 q 11除了包含编码细胞毒性丝氨酸蛋白酶B（CSP-B）、组织蛋白酶G和T细胞受体α和δ基因的基因外，还包括与CSP-B和组织蛋白酶G交叉杂交的额外基因组DNA克隆，即组织蛋白酶样基因-2（CGL-2）。本研究中报告的CSP-C cDNA克隆可能对应于CGL-2。
A human cDNA clone encoding a novel serine protease, cytotoxic serine protease‐C(CSP‐C), has been isolated from a cDNA library prepared from recombinant interleukin‐2 (IL‐2)‐activated lymphocytes of a patient with a large granular lymphoproliferative disorder. The clone has a 741‐base pair open reading frame encoding a putative 246‐amino acid protein. The protein sequence contains the catalytic charge relay system characteristic of a serine protease and the conserved N‐terminal amino acid sequence of the mature cytotoxic lymphocyte serine proteases found in both mouse and human. The amino acid sequence of CSP‐C has 71% identity with the previously reported cytotoxic serine protease‐B(CSP‐B)/human lymphocyte protease (HLP)/SECT and 57% identity with the granulocyte‐specific serine protease cathepsin G. The homology with another lymphocyte‐specific serine protease, human Hanukah factor (HF)/Granzyme A was 41%. The transcript is expressed in lymphocytes stimulated with IL‐2 or IL‐2 plus phytohemagglutinin (PHA). CSP‐C is not expresed in B‐lymphoblastoid cell lines or in the T‐leukemia cell line MOLT4. The cDNA sequence suggests that the protein is expressed as a prepropeptide, as has been found in the other murine and human serine proteases of lymphocyte origin. It has recently been reported that human chromosome 14q 11, in addition to containing the genes encoding cytotoxic serine protease B (CSP‐B), cathepsin G, and the T‐cell receptor alpha and delta genes, also includes an additional genomic DNA clone which cross‐hybridized with CSP‐B and cathepsin G, cathepsin‐like gene‐2 (CGL‐2). It is likely that the CSP‐C cDNA clone reported in this study corresponds to CGL‐2.