Alternatively spliced variants of Gallus gallus TNFRSF23 are expressed in the ovary and differentially regulated by cell signaling pathways
Alternatively spliced variants of Gallus gallus TNFRSF23 are expressed in the ovary and differentially regulated by cell signaling pathways
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DOI：
10.1095/biolreprod.103.023614
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发表时间：
2004-04-01
影响因子：
3.6
通讯作者：
Johnson, AL
中科院分区：
文献类型：
--
作者：
Bridgham, JT;Johnson, AL
作为搜索最近可用的鸡（ch）表达的序列标签数据库的结果，已经鉴定了与鼠（m）TNFRSF 23诱饵受体（DcR）相似的新的肿瘤坏死因子受体超家族（TNFRSF）成员。然而，与mTNFRSF 23相比，chTNFRSF 23存在至少两种剪接变体，其中一种包括死亡受体特有的细胞内死亡结构域（TNFRSF23.v1），另一种具有DcR的截短胞质结构域（命名为TNFRSF23.v2）。chTNFRSF 23的这两种剪接变体在各种母鸡组织中显示出mRNA表达的差异模式，在生殖组织中观察到最高水平。更具体地说，TNFRSF23.v1在卵巢排卵前卵泡颗粒细胞中表达最高，而TNFRSF23.v2 mRNA在卵巢基质组织中的水平最高。用颗粒细胞进行的原代培养实验确定TNFRSF23.v1 mRNA的表达通过蛋白激酶A信号传导而降低，并且通过转化生长因子（TGF）et处理而增强。有趣的是，TGF β 1和通过蛋白激酶C的信号传导也增强了TNFRSF23.v1的表达水平，但仅在来自前分层卵泡的未分化颗粒细胞中。基于mRNA表达模式及其内分泌/旁分泌调节，我们预测卵巢chTNFRSF 23代表颗粒细胞存活和/或分化的调节剂。最后，从进化的角度来看，这些受体变体的表征是相当感兴趣的，因为它们提供了额外的证据来支持TNFRSF成员在整个脊椎动物进化过程中的持续分化。
As a result of searching recently available chicken (ch) expressed sequence tag databases, a new Tumor Necrosis Factor Receptor Super Family (TNFRSF) member with similarity to the murine (m) TNFRSF23 decoy receptor (DcR) has been identified. However, by comparison with the mTNFRSF23, there exist at least two splice variants of chTNFRSF23, one of which includes an intracellular death domain (TNFRSF23.v1) characteristic of death receptors, and the other with a truncated cytoplasmic domain of a DcR (named TNFRSF23.v2). These two splice variants of chTNFRSF23 display differential patterns of mRNA expression across various hen tissues, with the highest levels observed within reproductive tissues. More specifically, TNFRSF23.v1 is most highly expressed in preovulatory follicle granulosa cells in the ovary, whereas TNFRSF23.v2 rnRNA is found at highest levels in ovarian stromal tissue. Primary culture experiments with granulosa cells determined that expression of TNFRSF23.v1 mRNA was decreased by protein kinase A signaling and enhanced by transforming growth factor (TGF) et treatment. Interestingly, TGFbeta1 and signaling via protein kinase C also enhanced levels of TNFRSF23.v1 expression but only in undifferentiated granulosa cells from prehierarchal follicles. Based on patterns of mRNA expression and its endocrine/paracrine regulation, we predict that ovarian chTNFRSF23 represents a modulator of granulosa cell survival and/or differentiation. Finally, the characterization of these receptor variants is of considerable interest from an evolutionary perspective in that they provide additional evidence to support a continuing divergence of TNFRSF members throughout vertebrate evolution.