Survival and photoreactivability of ultraviolet-irradiated cultured fish cells (CAF-MM1).
Survival and photoreactivability of ultraviolet-irradiated cultured fish cells (CAF-MM1).
复制标题
紫外线照射的培养鱼细胞 (CAF-MM1) 的存活率和光反应性。
DOI：
10.2307/3575489
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发表时间：
1980
影响因子：
3.4
通讯作者：
N. Egami
中科院分区：
文献类型：
--
作者：
Y. Mano;H. Mitani;H. Etoh;N. Egami
研究了鱼克隆细胞（caf1 - mm1）对紫外光（uv）的敏感性和光活化能力。利用菌落形成技术在不同辐射后温度（33、26和20/sup 0/C）下获得剂量-生存关系曲线。在26/sup 0/C时，D/sub 0/、D/sub q/和外推数(n)分别为1.74 J/m/sup 2/、2.62 J/m/sup 2/和4.5；在不同的温度下，这些数值没有明显的差异。紫外线照射后的可见光照射显著提高了存活率。咖啡因增加了caf1 - mm1细胞的紫外线敏感性，从结果来看，细胞具有一些咖啡因敏感的黑暗修复机制。
The sensitivity to ultraviolet light (uv) and photoreactivating ability of cultured fish clone cells (CAF-MM1) were investigated. Dose-survival relationship curves were obtained using the colony-forming technique at various postirradiation temperatures (33, 26, and 20/sup 0/C). At 26/sup 0/C the values of D/sub 0/, D/sub q/, and the extrapolation number (n) were 1.74 J/m/sup 2/, 2.62 J/m/sup 2/, and 4.5, respectively; no marked differences in these values were found among different temperatures. Visible light illumination after uv irradiation produced a marked increase in survival. No photoreactivation effects were observed beyond about 30 h. Caffeine increased uv sensitivity of the CAF-MM1 cells, and from the results it is suggested that the cells have some caffeine-sensitive dark repair mechanisms.