Nuclear Compartmentalization Is Abolished during Fission Yeast Meiosis
Nuclear Compartmentalization Is Abolished during Fission Yeast Meiosis
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DOI：
10.1016/j.cub.2010.09.004
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发表时间：
2010-11-09
期刊：
影响因子：
9.2
通讯作者：
Yamamoto, Masayuki
中科院分区：
文献类型：
--
作者：
Arai, Kunio;Sato, Masamitsu;Yamamoto, Masayuki
在真核细胞中，核膜将细胞核与细胞质分隔开。裂殖酵母粟酒裂殖酵母（Schizoschiomaceuspombe）经历封闭的有丝分裂，其中核被膜持续存在而不是像在高等真核细胞中那样被分解[1]。因此，假设核质运输在细胞周期中持续进行[2]。在这里，我们表明，核运输，事实上，废除特别是在后期的第二次减数分裂核分裂。在此期间，核质和细胞质蛋白都分散在整个细胞中，使人想起高等真核生物的开放有丝分裂，但S。粟酒孢核膜本身仍然存在。这种核质屏障的功能改变可能是由孢子壁形成引起的，因为孢子形成信号的异位诱导导致核质蛋白的过早分散。光漂白试验表明，核膜通透性突然增加，在第二次减数分裂后期的发病。当孢子形成被抑制时，通过阻断前孢子膜小泡从内质网到高尔基体的运输，渗透性没有改变。有证据表明，酵母配子发生产生囊泡运输介导的前孢子膜诱导核膜透化。
In eukaryotic cells, the nuclear envelope partitions the nucleus from the cytoplasm. The fission yeast Schizosaccharomyces pombe undergoes closed mitosis in which the nuclear envelope persists rather than being broken down, as in higher eukaryotic cells [1]. It is therefore assumed that nucleocytoplasmic transport continues during the cell cycle [2]. Here we show that nuclear transport is, in fact, abolished specifically during anaphase of the second meiotic nuclear division. During that time, both nucleoplasmic and cytoplasmic proteins disperse throughout the cell, reminiscent of the open mitosis of higher eukaryotes, but the architecture of the S. pombe nuclear envelope itself persists. This functional alteration of the nucleocytoplasmic barrier is likely induced by spore wall formation, because ectopic induction of sporulation signaling leads to premature dispersion of nucleoplasmic proteins. A photobleaching assay demonstrated that nuclear envelope permeability increases abruptly at the onset of anaphase of the second meiotic division. The permeability was not altered when sporulation was inhibited by blocking the trafficking of forespore-membrane vesicles from the endoplasmic reticulum to the Golgi. The evidence indicates that yeast gametogenesis produces vesicle transport-mediated forespore membranes by inducing nuclear envelope permeabilization.