Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase.
Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase.
复制标题
酵母鸟氨酸脱羧酶结构的生化和遗传表征。
DOI：
10.1016/0006-291x(89)90831-0
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Fonzi,WA
中科院分区：
文献类型：
--
作者：
Fonzi,WA
The ornithine decarboxylase gene ofS. cerevisiaeencodes a predicted protein of approximately 53 kD highly homologous with the ornithine decarboxylase of other species (1). However, the native enzyme has been reported as an 86 kD protein (2). Our molecular sieve analysis indicated a Mr=110,000 for the native enzyme. SDS-PAGE analysis of [H3]-α-difluoromethylornithine labelled enzyme demonstrated a subunit Mr of approximately 50 kD and suggested the native enzyme is a dimer. Genetic analyses support this conclusion. The complementary, ornithine decarboxylase deficient mutationsspe1A andspe1B were mapped to the enzyme structural gene by linkage analysis and gene conversion mapping. This demonstrated that the mutations exhibit intragenic complementation which suggests protein-protein interactions and an oligomeric structure for the yeast enzyme. We conclude that yeast ornithine decarboxylase is a dimeric enzyme of 53 kD subunits.
