INFLUENCE OF OXYGEN-DERIVED FREE-RADICAL SCAVENGERS ON ISCHEMIC LIVERS
INFLUENCE OF OXYGEN-DERIVED FREE-RADICAL SCAVENGERS ON ISCHEMIC LIVERS
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DOI：
10.1097/00007890-198512000-00002
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发表时间：
1985-01-01
期刊：
影响因子：
6.2
通讯作者：
CEDERNA, JP
中科院分区：
文献类型：
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作者：
ATALLA, SL;TOLEDOPEREYRA, LH;CEDERNA, JP
据推测，氧自由基的形成可能在缺血诱导的组织损伤中起重要作用。使用犬缺血性肝脏模型，我们已经评估了两种氧自由基清除剂，过氧化氢酶（CAT）和超氧化物歧化酶（SOD），单独使用和组合使用，对缺血性肝脏的恢复的作用。通过交叉夹闭门静脉和肝动脉40分钟，在成年杂种犬中诱导肝脏缺血。然后进行肝切除术，并在隔离灌注模型中使用500 ml白蛋白甘露醇溶液在37 ℃下进行3小时肝脏检测。在灌注期间每小时进行肝功能检查。灌注结束时行活检和99 m-Tc-希达显像。第1组（n= 6）中的肝脏作为对照，在缺血性损伤之前未进行预处理。第2组（n= 6）、第3组（n= 6）和第4组（n= 6）分别用CAT（5000 U/kg）、SOD（5000 U/kg）和SOD和CAT联合（5000 U/kg）预处理肝脏。结果表明，氧自由基清除剂CAT和SOD对缺血损伤时自由基的积累具有部分保护作用。这些研究为移植前后肝脏的保护提供了一些潜在的途径。
It has been hypothesized that the formation of oxygen-derived free radicals may play an important part in ischemically induced tissue injury. Using a canine ischemic liver model, we have assessed the role of two oxygen-free radical scavengers, catalase (CAT) and superoxide dismutase (SOD), used alone and in combination, on the recovery of ischemic livers. Liver ischemia was induced in adult mongrel dogs by cross-clamping of the portal vein and hepatic artery for 40 min. Hepatectomy was then performed, and livers were tested in an isolated perfusion model with 500 ml of an albuminmannitol solution at 37† C for 3 hr. Liver function tests were performed hourly during the perfusion period. Biopsies and 99m-Tc-HIDA scans were also done at the end of perfusion. Livers in group 1 (n= 6) served as controls and were not pretreated prior to ischemic injury. Livers in group 2 (n= 6), group 3 (n= 6), and group 4 (n= 6) were pretreated, respectively, with CAT (5000 U/kg), SOD (5000 U/kg), and SOD and CAT in combination (5000 U/kg) each. The results indicated that the oxygen-derived free radical scavengers, CAT and SOD, were able to provide partial protection against the free radicals accumulated during ischemic damage. These studies offer some potential avenues for the protection of livers prior to and after transplantation.