Effects of ketamine and pentobarbital on noradrenaline release from the medial prefrontal cortex in rats
Effects of ketamine and pentobarbital on noradrenaline release from the medial prefrontal cortex in rats
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DOI：
10.1007/bf03013235
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发表时间：
1999-04-01
期刊：
影响因子：
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通讯作者：
Matsuki, A
中科院分区：
文献类型：
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作者：
Kubota, T;Anzawa, N;Matsuki, A
目的：方法：在14只雄性Wistar大鼠的内侧前额叶皮质内植入一个带有2 mm长半透膜的微透析探针，用人工脑脊液以1 μ l·min（-1）的速率灌注透析探针。将大鼠随机分为氯胺酮组（K组，n = 7）和戊巴比妥组（P组，n=7）。随后在KI组中每只大鼠ip给予0（盐水）、1、10和100 mg.kg（-1）氯胺酮，在F1组中ip给予0（盐水）、0.5、5和50 mg/kg（-1）戊巴比妥。以20分钟的间隔通过具有电化学检测器的HPLC测量去甲肾上腺素浓度。（检测限：250 μ l（-1），测定的变异系数：4.9%）。结果：100 μ g氯胺酮（-1）后去甲肾上腺素释放mg.kg增加7.7 +/-2.0（SEM）μ g收集（-1），与盐水后2.7 +/-0.7，3.3 +/-1.0和4.2 +/-0.8 μ g收集（-1）相比，结论mg.kg：氯胺酮和戊巴比妥对内侧前额叶皮质去甲肾上腺素能神经元有不同的影响。氯胺酮对皮质去甲肾上腺素释放的刺激作用可能有助于氯胺酮麻醉独特的临床特征。
Purpose: To determine the effects of ketamine and pentobarbital on noradrenaline release from the medial prefrontal cortex.Methods: In 14 male Wistar rats, a microdialysis probe with a 2 mm long semipermeable membrane was implanted in the medial prefrontal colter, The dialysis probe was perfused at a rate of I mu l.min(-1) with an artificial cerebrospinal fluid solution. The rats were randomly allocated to two groups: ketamine (group K, n 7) and pentobarbital (group P, n=7). Each rat was subsequently given 0 (saline), 1, 10 and 100 mg.kg(-1) ketamine ip in group KI and 0 (saline), 0.5, 5 and 50 mg.kg(-1) pentobarbital ip in group Fl Sixty minutes elapsed between administration. Noradrenaline concentration was measured by HPLC with an electrochemical detector at 20 min intervals. (detection limit : 250 fg.20 mu l(-1), coefficient variation of the assay: 4.9%). The data in the 20-40 min after each dose of ketamine or pentobarbital ip were used for the statistical analysis.Results: Noradrenaline release after 100 mg.kg(-1) ketamine increased by 7.7 +/- 2.0 (SEM) pg.collection(-1) compared with 2.7 +/- 0.7, 3.3 +/- 1.0 and 4.2 +/- 0.8 pg.collection(-1) after saline, I and I 0 mg.kg(-1) ketamine, repectively (P < 0.05), Noradrenaline release did not change after pentobarbitalConclusion: This study suggests-the ketamine and pentobarbital have different effects on noradrenergic neurons in the medial prefrontal cortex. The stimulating effect of ketamine on noradrenaline release from the cortex might contribute to unique clinical features of ketamine anesthesia.