Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation
Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation
复制标题
电磁波照射的杀菌活性和口腔病原体灭活作用
DOI：
10.1111/j.1365-2672.2012.05307.x
复制
发表时间：
2012
影响因子：
4
通讯作者：
Takashi Matsuo
中科院分区：
文献类型：
--
作者：
Hiromichi Yumoto;Toshihiko Tominaga;Kouji Hirao;Tomoko Kimura;Kanako Takahashi;Takashi Sumitomo;Naoki Bando;Takashi Matsuo
Aims:The aim of this work was to clarify the effects of electromagnetic wave irradiation (EMWI) on oral bacterial pathogens.Methods and Results:A Gram‐negative (Porphyromonas gingivalis) or Gram‐positive (Streptococcus mutans,S. intermedius,Enterococcus faecalis) bacterial suspension was irradiated by EMW apparatus (500–1000 kHz, 5–15 times, 1 s time−1). Quantification of survival bacteria by CFU counting revealed that EMWI exhibited marked bactericidal activity against all tested bacteria and bactericidal activity at 500 kHz increased in an irradiation number‐dependent manner. After EMWI at 500 kHz, scanning electron microscopic observations showed that the chain ofS. mutanscells was shortened after 5 irradiations and the outlines of bacterial cells (S. mutansandP. gingivalis) were unclear after 5–10 irradiations. EMWI inhibited the inductive effect ofS. mutanson pro‐inflammatory cytokine production in human monocytes and this inhibitory effect was comparable with that of heat‐killed bacteria. Furthermore, using an enzyme activity assay, EMWI partially inactivated the activities of gingipains fromP. gingivalis.Conclusions:These findings demonstrated that EMWI has inactivation and bactericidal activities against single microbial species among four kinds of oral pathogens.Significance and Impact of the Study:Electromagnetic wave irradiation may be applicable for medical disinfection and sterilization, such as refractory periapical periodontitis.
