Properties of the viruses selected during persistent infection of L cells with VSV.
Properties of the viruses selected during persistent infection of L cells with VSV.
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VSV 持续感染 L 细胞期间选择的病毒的特性。
DOI：
10.1099/0022-1317-40-2-481
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
K. Shimokata
中科院分区：
文献类型：
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作者：
Y. Nishiyama;Y. Ito;K. Shimokata
从新泽西型VSV持续感染的L细胞中分离到两个病毒克隆，即VSV-mp和VSV-sp。两个克隆都比亲本病毒VSV-0对温度更敏感，生长更慢，产生更小的噬斑，更小的c.p.e.。并且在L细胞中病毒产量较低。与亲本病毒不同，这两种持久性病毒均诱导L细胞产生干扰素。无需干扰素预处理，即可从低倍性感染VSV-sp的L细胞中获得稳定的载体培养物。这可能与VSV-sp比VSV-mp或VSV-o对干扰素更敏感有关。
Two virus clones, VSV-mp and VSV-sp, were isolated from L cells persistently infected with VSV (New Jersey serotype). Both clones were more temperature sensitive than the parent virus, VSV-o, and grew more slowly, gave smaller plaques, less c.p.e. and lower virus yields in L cells. Unlike the parent virus, both persistent viruses induced interferon production in L cells. Stable carrier cultures could be obtained from L cells infected with VSV-sp at low multiplicities without pretreatment with interferon. This may be related to the fact that VSV-sp is more sensitive to interferon than either VSV-mp or VSV-o.