Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1
Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1
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DOI：
10.1155/2004/204953
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发表时间：
2004-10-01
期刊：
影响因子：
2.4
通讯作者：
Imanaka, Tadayuki
中科院分区：
文献类型：
--
作者：
Atomi, Haruyuki;Fukui, Toshiaki;Imanaka, Tadayuki
从日本Kodakara岛上的一种solfatara中分离出一种超嗜热古菌菌株，KOD 1，以前曾被报道为Pyrococcus sp. KOD 1。然而，一个详细的系统发育树，可能通过最近积累的16 S rRNA序列的各种物种的顺序Thermococcales，表明菌株KOD 1是一个成员的属Thermococcus。我们进行了DNA-DNA杂交试验，对物种，表现出高度相似的16 S核糖体DNA序列，包括嗜胨热球菌和嗜热球菌斯氏。杂交结果以及生长特性和底物利用的差异将菌株KOD 1与嗜胨嗜热厌氧杆菌（Tpeptonophilus）和斯氏嗜热厌氧杆菌（Tstetteri）在种水平上区分开来。结果表明，KOD 1菌株代表一个新的热球菌属种，命名为Thermococcus kodakaraensis KOD 1 sp. nov.
A hyperthermophilic archaeal strain, KOD1, isolated from a solfatara on Kodakara Island, Japan, has previously been reported as Pyrococcus sp. KOD1. However, a detailed phylogenetic tree, made possible by the recent accumulation of 16S rRNA sequences of various species in the order Thermococcales, indicated that strain KOD1 is a member of the genus Thermococcus. We performed DNA-DNA hybridization tests against species that displayed high similarity in terms of 16S ribosomal DNA sequences, including Thermococcus peptonophilus and Thermococcus stetteri. Hybridization results and differences in growth characteristics and substrate utilization differentiated strain KOD1 from T peptonophilus and T stetteri at the species level. Our results indicate that strain KOD1 represents a new species of Thermococcus, which we designate as Thermococcus kodakaraensis KOD1 sp. nov.