THE EFFECTS OF SELECTIVE MATRIX DEGRADATION ON THE SHORT-TERM COMPRESSIVE PROPERTIES OF ADULT HUMAN ARTICULAR-CARTILAGE
THE EFFECTS OF SELECTIVE MATRIX DEGRADATION ON THE SHORT-TERM COMPRESSIVE PROPERTIES OF ADULT HUMAN ARTICULAR-CARTILAGE
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DOI：
10.1016/0304-4165(92)90111-7
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发表时间：
1992-04-22
期刊：
影响因子：
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通讯作者：
BARRETT, AJ
中科院分区：
文献类型：
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作者：
BADER, DL;KEMPSON, GE;BARRETT, AJ
蛋白多糖和胶原蛋白消化对人体关节软骨在无侧限压缩测试时的瞬态响应的影响进行了测定。从髋关节的股骨头和膝关节的股骨髁分离的小圆柱形软骨样本，使用设计用于产生瞬态振荡响应的测试装置经受突然施加的压缩载荷。由此确定了弹性刚度和粘性阻尼系数的响应值。组织蛋白酶D和组织蛋白酶B1用于消化某些标本中的蛋白多糖，而在其他标本中，白细胞弹性蛋白酶用于攻击II型原胶原分子的非螺旋末端区域，可能还有IX型胶原分子，从而干扰胶原网的完整性。结果表明，单独的蛋白聚糖消化降低了粘性阻尼系数，但它没有显着改变的弹性刚度确定的振荡响应。相反，弹性蛋白酶的作用降低了软骨的阻尼系数和弹性刚度。结果表明蛋白聚糖在调节软骨中的流体运输中的作用，从而控制时间依赖性粘性特性。弹性刚度被证明是依赖于胶原纤维网络的完整性，而不是蛋白聚糖。
The effects of proteoglycan and collagen digestion on the transient response of human articular cartilage when tested in unconfined compression were determined. Small cylindrical specimens of cartilage, isolated from the femoral head of the hip joint and from the femoral condyles of the knee joint, were subjected to a suddenly applied compressive load using a test apparatus designed to yield a transient oscillatory response. From this response values of the elastic stiffness and the viscous damping coefficient were determined. Cathepsin D and cathepsin B1 were used to digest the proteoglycan in some specimens, while in other specimens leukocyte elastase was used to attack the non-helical terminal regions of the Type II tropocollagen molecules and possibly the Type IX collagen molecule and thereby disturb the integrity of the collagen mesh. The results showed that proteoglycan digestion alone reduced the viscous damping coefficient but it did not significantly alter the elastic stiffness as determined from the oscillatory response. In contrast, the action of elastase reduced both the damping coefficient and the elastic stiffness of the cartilage. The results demonstrated the role of proteoglycans in regulating fluid transport in cartilage and hence controlling the time-dependent viscous properties. The elastic stiffness was shown to be dependent on the integrity of the collagen fibre network and not on the proteoglycans.