Novel membrane-permeable contrast agent for brain tumor detection by MRI.
Novel membrane-permeable contrast agent for brain tumor detection by MRI.
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DOI：
10.1002/mrm.22216
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发表时间：
2010-03
影响因子：
3.3
通讯作者：
Moore, Anna
中科院分区：
文献类型：
--
作者：
Kumar, Mohanraja;Medarova, Zdravka;Pantazopoulos, Pamela;Dai, Guangping;Moore, Anna
阻碍脑癌临床干预的关键挑战之一是无法在足够早的阶段检测到脑肿瘤以进行有效的治疗。此外，这种癌症的快速生长和严重致死率预示着监测治疗干预后病理学发展及其结果的至关重要性。考虑到这一点，我们设计了一种新型膜渗透造影剂 MN-MPAP-Cy5.5，它由超顺磁性氧化铁核心组成，用于与肉豆蔻酰化聚精氨酸肽缀合的 MRI，作为膜易位模块，并用近红外染料 Cy5.5 标记用于相关显微镜检查。该探针在体外显示出 U-87 人神经胶质瘤细胞的显着摄取，并通过 MRI 在体内定位和描绘立体定向注射的肿瘤。我们的研究结果表明，该药物通过磁性纳米颗粒标签在渗漏的肿瘤脉管系统中易位，然后在肿瘤细胞中增强积累来介导其作用。从成像的角度来看，当脑肿瘤还很小时，对其进行无创检测是一个巨大的挑战。我们的研究描述了一种改进的策略，通过利用具有膜易位特性的造影剂来检测脑损伤。
One of the key challenges hindering the clinical intervention against brain cancer is defined by the inability to detect brain tumors at an early enough stage to permit effective therapy. Furthermore, the rapid growth and severe lethality of this form of cancer predicate the vital importance of monitoring the development of the pathology and its outcome after therapeutic intervention. With this in mind, we designed a novel membrane-permeant contrast agent, MN-MPAP-Cy5.5, which consists of a superparamagnetic iron oxide core, for MRI conjugated to myristoylated polyarginine peptides, as a membrane translocation module and labeled with the near-infrared dye Cy5.5 for correlative microscopy. This probe showed a remarkable uptake by U-87 human glioma cells in vitro and localized and delineated stereotactically injected tumor in vivo by MRI. Our findings suggest that the agent mediates its effects by translocation of the magnetic nanoparticles label across the leaky tumor vasculature, followed by enhanced accumulation in tumor cells. The noninvasive detection of brain tumors when they are still small represents a formidable challenge from an imaging standpoint. Our study describes an improved strategy to detect brain lesions by utilizing a contrast agent with membrane translocation properties.
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影响因子：
15.9
作者：
Fadok, VA;Bratton, DL;Henson, PM
通讯作者：
Henson, PM
影响因子：
5
作者：
Neuwelt, EA;Várallyay, P;Nixon, R
通讯作者：
Nixon, R
DOI：
10.1016/j.ijrobp.2004.12.026
发表时间：
2005-03-15
影响因子：
7
作者：
Adam, JF;Biston, MC;Elleaume, H
通讯作者：
Elleaume, H
影响因子：
6.7
作者：
BAXTER, AB;MELNIKOFF, S;BRASCH, RC
通讯作者：
BRASCH, RC
影响因子：
11.5
作者：
Leenders, WPJ;Küsters, B;de Waal, R
通讯作者：
de Waal, R