Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. IV. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes.
Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. IV. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes.
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由体细胞杂交体衍生的抗体定义的人类淋巴细胞标记物。
DOI：
10.4049/jimmunol.126.4.1373
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发表时间：
1981
影响因子：
4.4
通讯作者：
H. Zola
中科院分区：
文献类型：
--
作者：
D. Brooks;I. Beckman;J. Bradley;P. Mcnamara;M. Thomas;H. Zola
体细胞杂交技术已用于生产针对人B淋巴母细胞系HRIK的单克隆抗体（命名为FMC 7）。通过间接免疫荧光，FMC 7与10个B细胞系中的总共3个反应，但不能结合T和T淋巴细胞系。在正常外周血中，FMC 7结合3 - 6%的单核细胞。当正常的外周血单核细胞进行分级，FMC 7只与B-丰富的部分，染色的一些，但不是所有的细胞表达SMIg。双标记物分析表明，FMC 7检测到的抗原与任何常规B细胞标记物都不相同，包括表面膜IgM、C3受体、Fc受体和小鼠红细胞的结合位点。另一种与B淋巴细胞特异性反应的单克隆抗体FMC 1结合一个单独的抗原决定簇。因此，FMC 7似乎识别特异性存在于人B淋巴细胞亚群上的先前未检测到的标志物。FMC 7与一些但不是所有的B细胞白血病反应，并且可能在白血病中具有诊断应用。
The technique of somatic cell hybridization has been used to produce a monoclonal antibody (designated FMC7) against the human B lymphoblastoid cell line HRIK. By indirect immunofluorescence FMC7 reacted with a total of 3 out of 10 B cell lines, but failed to bind T and Null cell lines. On normal peripheral blood, FMC7 bound between 3 and 6% of mononuclear cells. When normal peripheral blood mononuclear cells were fractionated, FMC7 reacted with only the B-enriched fraction, staining some but not all cells expressing SMIg. Double marker analysis indicated that the antigen detected by FMC7 is not identical with any of the conventional B cell markers, including surface membrane IgM, C3 receptors, Fc receptors, and the binding site for mouse erythrocytes. Another monoclonal antibody, FMC1, which reacts specifically with B lymphocytes, binds a separate antigenic determinant. Thus, FMC7 appears to recognize a previously undetected marker present specifically on a subpopulation of human B lymphocytes. FMC7 reacts with some but not all B cell leukemias and may have diagnostic applications in leukemia.