Genetic diversity and population structure of Plasmodium falciparum isolates from Dakar, Senegal, investigated from microsatellite and antigen determinant loci
Genetic diversity and population structure of Plasmodium falciparum isolates from Dakar, Senegal, investigated from microsatellite and antigen determinant loci
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DOI：
10.1016/s1286-4579(02)01587-3
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发表时间：
2002-06-01
影响因子：
5.8
通讯作者：
Renaud, F
中科院分区：
文献类型：
--
作者：
Leclerc, MC;Durand, P;Renaud, F
利用两种不同类型的分子标记、19个微卫星和4个抗原决定簇，对32份恶性疟原虫血样分离株(25株来自达喀尔市及郊区，7株来自塞内加尔其他地区)的遗传多样性和群体结构进行了研究。在相同的技术程序下，微卫星座位的等位基因平均数高于抗病基因座。两项标记物均显示15.6%的血样为多重感染。根据微卫星和抗原计算的平均期望杂合度相似，分别为0.74和0.70。微卫星座位和抗原决定簇之间存在显著的连锁不平衡。这表明了该样本的一种非万能结构，可以用两个非排他性假设来解释：(I)特定的交配系统(即克隆)，和/或(Ii)恶性疟原虫的种群结构(即瓦伦德效应)。城市样本可能来自寄生虫传播程度不同的一组不同的疫源地。此外，多位点基因分型与不同参数(如年龄、性别、寄生虫病患者的血型、每微升血液中滋养体的数量)无关。对这些结果的讨论考虑到了最近发表的关于疟疾种群生物学的研究。(C)2002年版《爱思唯尔科学与医学》。版权所有。
We investigated the genetic diversity and the population structure of 32 Plasmodium falciparum blood sample isolates (25 from Dakar city and suburbs and seven from other localities in Senegal) with two different types of molecular markers, 19 microsatellite and four antigenic determinant loci. Under the same technical procedure, microsatellite loci showed a mean number of alleles greater than that of anticenic loci. Both markers revealed that 15.6% of blood samples were multi-infected. Mean expected heterozygosity calculated from microsatellites and antigens was similar, 0.74 and 0.70, respectively. Significant linkage disequilibrium was observed from microsatellite loci and antigenic determinant loci. This suggests a non-panmictic structure for this sample that could be explained by two non-exclusive hypotheses: (i) a particular mating system (i.e. clonality), and/or (ii) a population structure in P. falciparum (i.e. Wahlund effect). Urban samples could have been drawn from a heterogeneous set of foci with different level of parasitic transmission. Moreover, no relationship was found between multilocus genotypes and different parameters (i.e. age, sex and blood group of parasitized patients number of trophozoites per microliter of blood). The results are discussed taking into account recently published Studies on malaria population biology. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.