Effect of stress on growth, pigment production and morphology of Monascus sp in solid cultures
Effect of stress on growth, pigment production and morphology of Monascus sp in solid cultures
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DOI：
10.1002/jobm.200610261
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发表时间：
2007-04-01
影响因子：
3.1
通讯作者：
Pandey, Ashok
中科院分区：
文献类型：
--
作者：
Babitha, Sumathy;Soccol, Carlos Ricardo;Pandey, Ashok
本工作的目的是研究胁迫对红曲霉固态发酵产色素的影响。通过在不同的高温下孵育培养物来诱导热应激。为了施加渗透胁迫，在高压灭菌前向固体基质中添加不同浓度的氯化钠和甘油。用光学显微镜和扫描电子显微镜进行了形态观察。当细胞暴露在高温、高甘油和高盐浓度下时，细胞色素的产生和生长发生了显著的变化。高温(>；45摄氏度)会导致更多黄色色素的产生。高浓度的氯化钠诱导分生孢子，使真菌生物量下降50%，但红色素产量从11.86OD/gDS增加到20.14OD/gds。当受到甘油胁迫时，气生菌丝体显著增加。与对照条件比较时观察到。研究结果对开发紫红曲霉LPB 97真菌培养物生产红色、黄色或大幅度提高红色素产量具有重要意义。因此，这些结果可能会很好地影响用于工业应用的红曲色素的商业方面。
The aim of the current work was to study the influence of stress on pigment production in Monascus purpureus under solid-state fermentation. Thermal stress was induced by incubating the culture at various high temperatures. For giving osmotic stress, different concentration of NaCl and glycerol were added to the solid substrate prior to autoclaving. Morphological studies were done by light microscopy and scanning electron microscopy. When the cells were exposed to high temperature, high glycerol and salt concentration, significant changes in pigment production and growth were observed. High temperature (>45 degrees C) induced the production of more yellow pigments. High concentration of NaCl induced conidiation and caused a decrease in fungal biomass (up to 50%) but red pigment production increased from 11.86 OD/gds to 20.14 OD/gds. When subjected to glycerol stress, a significant increase in aerial mycelia. was observed when compared with the control conditions. The results attain significance for exploiting the fungal culture of Monascus purpureus LPB 97 for producing colors of choice- red, or yellow, or increasing the yield of red pigments considerably. Thus, these results could well impact the commercial aspect of Monascus pigments for industrial application.