Assignment of ESR signals of Escherichia coli terminal oxidase complexes.
Assignment of ESR signals of Escherichia coli terminal oxidase complexes.
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大肠杆菌末端氧化酶复合物的 ESR 信号分配。
DOI：
10.1016/0005-2728(85)90206-3
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Y. Anraku
中科院分区：
文献类型：
--
作者：
A. Hata;Y. Kirino;K. Matsuura;S. Itoh;T. Hiyama;K. Konishi;K. Kita;Y. Anraku
在液氦温度下，对大肠杆菌需氧呼吸链各主要成分的ESR信号进行了测定和归属。Cytochromeb-556给出了一个微弱的高自旋信号ATG=6.0。末端氧化酶细胞色素b-562·o复合体给出信号atg=6.0、3.0和2.26，末端氧化酶细胞色素b-558·d复合体给出信号atg=6.0、2.5和2.3。在纯化的细胞色素b-562·o络合物中观察到来自铜离子的信号，接近g=2.0。在空气氧化条件下，KCN或NaN_3对细胞色素的影响表明，细胞色素具有高自旋的血红素，而细胞色素具有低自旋的血红素。用ESR信号的电位滴定测定，细胞色素B-558和细胞色素B-558的ESR信号在膜制剂中分别为140 mV和240 mV，在纯化制剂中为30 mV和240 mV。氧化后的细胞色素产生强烈的低自旋信号，ATG=2.5和2.3，而空气氧化条件下的细胞色素只发出很低强度的信号。这些结果表明，在空气氧化条件下，大多数细胞色素含有一个带有结合氧的抗磁性铁原子。
The ESR signals of all the major components of the aerobic respiratory chain ofEscherichia coliwere measured and assigned at liquid helium temperature. Cytochromeb-556 gives a weak high-spin signal atg= 6.0. The terminal oxidase cytochromeb-562 ·ocomplex gives signals atg= 6.0, 3.0 and 2.26, and the terminal oxidase cytochromeb-558 ·dcomplex gives signals atg= 6.0, 2.5 and 2.3. A signal derived from cupric ions in the purified cytochromeb-562 ·ocomplex was observed nearg= 2.0. It was shown by the effects of KCN or NaN3on cytochromes under the air-oxidized conditions that cytochromeohas a high-spin heme and cytochromedhas a low-spin heme. TheE′mvalues for cytochromesb-558 andd, respectively, determined by potentiometric titration of the ESR signals were 140 and 240 mV in the membrane preparation, and 30 and 240 mV in the purified preparation. The oxidized cytochromedgave intense low-spin signals atg= 2.5 and 2.3, while cytochromedunder the air-oxidized conditions gave corresponding signals of only very low intensity. These results suggested that most of the cytochromedunder the air-oxidized conditions contains a diamagnetic iron atom with a bound dioxygen.
由需氧生长的大肠杆菌 K12 制备的多层膜中细胞色素的方向。
DOI：
10.1099/00221287-119-1-145
发表时间：
1980
期刊：
Journal of general microbiology
影响因子：
--
作者：
Poole,RK;Blum,H;Scott,RI;Collinge,A;Ohnishi,T
通讯作者：
Ohnishi,T