Effect of saliva viscosity on the co-aggregation between oral streptococci and Actinomyces naeslundii
Effect of saliva viscosity on the co-aggregation between oral streptococci and Actinomyces naeslundii
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DOI：
10.1111/j.1741-2358.2011.00595.x
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发表时间：
2012-06-01
期刊：
影响因子：
2
通讯作者：
Oho, Takahiko
中科院分区：
文献类型：
--
作者：
Kitada, Katsuhiro;Oho, Takahiko
唾液粘度对口腔链球菌和内氏放线菌共聚集的影响背景：口腔细菌的共聚集导致它们从口腔中清除。老年人口腔卫生不良、唾液粘度高是常见现象；目的：探讨唾液粘度对口腔链球菌与放线菌共聚集的直接影响。材料与方法：使用15株口腔链球菌和单一放线菌菌株。通过磷酸盐缓冲液中的视觉测定和含有 0-60% 甘油或全唾液的相同缓冲液中的分光光度测定来评估共聚集。结果：在视觉测定中，九种口腔链球菌与内氏放线菌 ATCC12104 共聚集，随后用于分光光度分析。所有测试的菌株都显示出随着缓冲液中甘油含量的增加，共聚集减少。去除甘油后，口腔链球菌与内氏放线菌的共聚集恢复至基线水平。口腔链球菌与内氏放线菌的共聚集百分比与未刺激和刺激的全唾液样本的粘度显着相关（相关系数分别为 0.52 和 0.48）。结论：本研究表明唾液粘度影响口腔链球菌与放线菌的共聚集，并且随着唾液粘度的增加，细菌共聚集减少。
Effect of saliva viscosity on the co-aggregation between oral streptococci and Actinomyces naeslundiiBackground: The co-aggregation of oral bacteria leads to their clearance from the oral cavity. Poor oral hygiene and high saliva viscosity are common amongst the elderly; thus, they frequently suffer from pneumonia caused by the aspiration of oral microorganisms.Objectives: To examine the direct effect of saliva viscosity on the co-aggregation of oral streptococci with actinomyces.Materials and methods: Fifteen oral streptococcal and a single actinomyces strain were used. Coaggregation was assessed by a visual assay in phosphate buffer and a spectrophotometric assay in the same buffer containing 0-60% glycerol or whole saliva.Results: Nine oral streptococci co-aggregated with Actinomyces naeslundii ATCC12104 in the visual assay and were subsequently used for the spectrophotometric analysis. All tested strains displayed a decrease in co-aggregation with increasing amounts of glycerol in the buffer. The co-aggregation of Streptococcus oralis with A. naeslundii recovered to baseline level following the removal of glycerol. The per cent co-aggregation of S. oralis with A. naeslundii was significantly correlated with the viscosity in unstimulated and stimulated whole saliva samples (correlation coefficients:) 0.52 and) 0.48, respectively).Conclusion: This study suggests that saliva viscosity affects the co-aggregation of oral streptococci with actinomyces and that bacterial co-aggregation decreases with increasing saliva viscosity.