Effects of animal density, volume, and the use of 2D/3D recording on behavioral studies of copepods
Effects of animal density, volume, and the use of 2D/3D recording on behavioral studies of copepods
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DOI：
10.1007/s10750-010-0586-z
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发表时间：
2011-05-01
期刊：
影响因子：
2.6
通讯作者：
Hwang, Jiang-Shiou
中科院分区：
文献类型：
--
作者：
Dur, Gael;Souissi, Sami;Hwang, Jiang-Shiou
桡足类行为的研究既需要适当的实验设计，也需要进行客观可验证的数值分析的手段。尽管关于桡足类行为的出版物越来越多，但很难比较这些研究。本研究以两种桡足类-Eurytemora affinis和Pseudodiaptomus annandalei为研究对象，采用最新发展的定标和非定标方法研究了密度和体积对静水中个体生物游动行为的影响。我们还比较了游泳轨迹的二维和三维投影的结果。规模依赖和规模独立的分析相结合，发现非常有效地描述了一些行为观察。我们发现，（i）密度没有影响，除了增加在游泳状态下的时间“打破”，（ii）较小的体积导致更复杂的轨迹，和较大的体积，如密度，增加了在游泳状态下的时间“打破”，和（iii）三维投影给出了一个更准确的估计速度和巡航时间。当只使用垂直2D投影时，“巡航”可能与“下沉”混淆。这些结果表明，实验条件和2D或3D投影的选择对于桡足类行为的研究具有重要意义。制定标准化程序，用以比较不同研究报告中的观察结果，是一个特别紧迫的问题。
Studies on the behavior of copepods require both an appropriate experimental design and the means to perform objectively verifiable numerical analysis. Despite the growing number of publications on copepod behavior, it has been difficult to compare these studies. In this study, we studied two species of copepods, Eurytemora affinis and Pseudodiaptomus annandalei, and employed recently developed scaling and non-scaling methodology to investigate the effects of density and volume on the swimming behavior of individual organisms in still water. We also compared the results of two- and three-dimensional projections of the swimming tracks. A combination of scale-dependent and scale-independent analysis was found to characterize a number of behavioral observations very effectively. We discovered that (i) density has no effect except to increase the time spent in the swimming state of "breaking", (ii) smaller volumes resulted in more complex trajectories, and larger volumes, like density, increased the time spent in the swimming state "breaking", and (iii) three-dimensional projections gave a more accurate estimation of speed and the time spent cruising. When only a vertical 2D projection was used, "cruising" could be confused with "sinking". These results indicate that both experimental conditions and the selection of 2D or 3D projection have important implications regarding the study of copepod behavior. The development of standardized procedures with which to compare the observations made in different studies is an issue of particular urgency.