Using a modified standard microscope to generate virtual slides.
Using a modified standard microscope to generate virtual slides.
复制标题
使用改进的标准显微镜生成虚拟载玻片。
DOI：
10.1002/ar.b.10017
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Ayers,LeonaW
中科院分区：
文献类型：
--
作者：
Romer,DavidJ;Yearsley,KurtisH;Ayers,LeonaW
通过添加带有H29控制器和0.1-μm线性标尺的Prior Scientific H101机器人载物台以及Hitachi HV-C20 3CCD相机，将标准显微镜重新配置为虚拟载玻片生成器。Media Cybernetics Image Pro Plus版本4（IP 4）软件控制载物台在X、Y和Z轴上的移动，而Media Cybernetics Pro系列捕获套件捕获640 × 480像素的图像。载物台校准、扫描算法、存储要求和查看模式都已标准化。使用IP 4将捕获的图像剪辑成大型虚拟幻灯片图像，随后以TIF或JPEG格式保存。在工作站使用IP 4查看器以及Adobe Photoshop和Kodak Imaging查看虚拟切片。MGI Zoom Server将虚拟载玻片传输到互联网上，Microphilfield的Neuroinformatica查看软件提供了基于浏览器的虚拟显微镜界面以及用于注释虚拟载玻片的标记工具。这些图像是从Windows 2000平台提供的，该平台具有2 GB RAM、500 GB磁盘存储和1.0 GHz P4处理器。为了节省图像服务器上的磁盘空间，使用10：1的压缩比将TIF文件转换为FlashPix（FPX）文件格式。通过使用4×、10×、20×和40×物镜，现在可以生成具有高质量和形态细节的组织整体切片和组织阵列的非常大的千兆像素图像，用于教学、出版、研究和形态测量分析。我们的系统的技术细节和演示可以在http://virtualmicroscope.osu.edu网站上找到。Anat Rec（Part B：New Anat）272 B：91-97，2003年。© 2003 Wiley利斯公司
A standard microscope was reconfigured as a virtual slide generator by adding a Prior Scientific H101 robotic stage with H29 controller and 0.1‐μm linear scales and a Hitachi HV‐C20 3CCD camera. Media Cybernetics Image Pro Plus version 4 (IP4) software controlled stage movement in the X‐, Y‐, and Z‐axis, whereas a Media Cybernetics Pro‐Series Capture Kit captured images at 640 × 480 pixels. Stage calibration, scanning algorithms, storage requirements, and viewing modes were standardized. IP4 was used to montage the captured images into a large virtual slide image that was subsequently saved in TIF or JPEG format. Virtual slides were viewed at the workstation using the IP4 viewer as well as Adobe Photoshop and Kodak Imaging. MGI Zoom Server delivered the virtual slides to the Internet, and MicroBrightField's Neuroinformatica viewing software provided a browser‐based virtual microscope interface together with labeling tools for annotating virtual slides. The images were served from a Windows 2000 platform with 2 GB RAM, 500 GB of disk storage, and a 1.0 GHz P4 processor. To conserve disk space on the image server, TIF files were converted to the FlashPix (FPX) file format using a compression ratio of 10:1. By using 4×, 10×, 20×, and 40× objectives, very large gigapixel images of tissue whole‐mounts and tissue arrays with high quality and morphologic detail are now being generated for teaching, publication, research, and morphometric analysis. Technical details and a demonstration of our system can be found on the Web at http://virtualmicroscope.osu.edu. Anat Rec (Part B: New Anat) 272B:91–97, 2003. © 2003 Wiley‐Liss, Inc.