Developmental changes in the intensity and distribution pattern of green fluorescence in coral larvae and juveniles
Developmental changes in the intensity and distribution pattern of green fluorescence in coral larvae and juveniles
复制标题
珊瑚幼体和幼体绿色荧光强度和分布模式的发育变化
DOI：
10.3755/galaxea.21.1_13
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
M. Hidaka
中科院分区：
文献类型：
--
作者：
D. Haryanti;M. Hidaka
由于绿色荧光蛋白（GFP），珊瑚幼虫和幼虫经常表现出绿色荧光。珊瑚绿色荧光分布模式的个体发生变化尚不完全清楚。我们研究了细角鹿角珊瑚、大角珊瑚和帕利弗珊瑚在早期发育阶段绿色荧光强度和分布模式的变化。研究的三个物种幼虫的绿色荧光分布不同，并且在幼虫变态为原发息肉后发生变化。绿色荧光主要分布在P. damicornis 和I. palifera 幼虫的表皮中，而在A. tenuis 幼虫中则分布在胃真皮中。在 P. damicornis 和 A. tenuis 原发性息肉中，口腔开口周围和触手环下方的环形结构中存在绿色荧光。 P. damicornis 和 I. palifera 原代息肉的触手尖端也存在绿色荧光。此外，无论共生体获取是否成功，在接种共生体后，A. tenuis 原发息肉中的绿色荧光强度显着增加。在 I. palifera 中，荧光强度降低，在某些情况下，在获得共生体后甚至消失。此外，漂白胁迫增加了 P. damicornis 幼鱼的绿色荧光。目前的观察表明，幼年珊瑚的绿色荧光强度受到共生细胞的存在或获得以及压力条件的影响。这项研究还表明，GFP 在珊瑚物种之间以及珊瑚的不同发育阶段可能具有不同的功能。
Coral larvae and juveniles often exhibit green fluorescence due to green fluorescence proteins (GFP). Ontogenetic changes in the distribution pattern of green fluorescence in coral are not fully understood. We investigated changes in the intensity and distribution pattern of green fluorescence of the corals Acropora tenuis, Pocillopora damicornis, and Isopora palifera during early developmental stages. Green fluorescence distribution in larvae differed among the three species studied and changed after the larvae metamorphosed into primary polyps. Green fluorescence distributed mainly in the epidermis of P. damicornis and I. palifera larvae but in the gastrodermis in A. tenuis larvae. In P. damicornis and A. tenuis primary polyps, green fluorescence was present around the oral opening and in a ring structure below the tentacular ring. Green fluorescence was also present on the tips of the tentacles of P. damicornis and I. palifera primary polyps. Furthermore, green fluorescence intensity in A. tenuis primary polyps increased significantly after inoculation of symbionts, irrespective of the success of symbiont acquisition. In I. palifera, the fluorescence in tensity decreased and, in some cases, disappeared after the acquisition of symbionts. Moreover, bleaching stress increased green fluorescence in P. damicornis juveniles. The present observations suggest that green fluorescence intensity in juvenile corals is influenced by the presence or acquisition of symbiont cells as well as by stressful conditions. This study also suggests that GFP might have different functions among coral species and at different developmental stages of the corals.
影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV